Anticorps Polyclonal de lapin anti-AGO2

AGO2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, RIP, IP, CLIP, ELISA

Conjugaison

Non conjugué

Publications

63

Avis (1)

N° de cat : 10686-1-AP

Synonymes

AGO2, Argonaute2, eIF 2C 2, eIF2C 2, EIF2C2, hAgo2, PAZ Piwi domain protein, PPD, Protein argonaute 2, Protein slicer, Q10

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Galerie de données de validation

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  WB analysis of HEK-293 using 10686-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10686-1-AP (AGO2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10686-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10686-1-AP (AGO2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IP: See 6 publications below
• RIP: See 33 publications below

Informations sur le produit

10686-1-AP cible AGO2 dans les applications de WB, RIP, IP, CLIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AGO2 Protéine recombinante Ag1032
• Nom complet: eukaryotic translation initiation factor 2C, 2
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 94-97 kDa
• Numéro d’acquisition GenBank: BC007633
• Symbole du gène: EIF2C2
• Identification du gène (NCBI): 27161
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions. This antibody is specific to EIF2C2. EIF2C2 exists two isoforms and molecular weight of isoforms are 97 kDa and 94 kDa.

Publications

Species	Application	Title
		Nat Med; Cancer cells exploit an orphan RNA to drive metastatic progression.; Authors - Lisa Fish
human	IP	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	Nat Commun; Liver governs adipose remodelling via extracellular vesicles in response to lipid overload.; Authors - Yue Zhao
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
human	RIP	J Hematol Oncol; LINC01123, a c-Myc-activated long non-coding RNA, promotes proliferation and aerobic glycolysis of non-small cell lung cancer through miR-199a-5p/c-Myc axis.; Authors - Qian Hua
	RIP	Clin Transl Med; LncRNA nuclear-enriched abundant transcript 1 shuttled by prostate cancer cells-secreted exosomes initiates osteoblastic phenotypes in the bone metastatic microenvironment via miR-205-5p/runt-related transcription factor 2/splicing factor proline- and glutamine-rich/polypyrimidine tract-binding protein 2 axis.; Authors - Chengqiang Mo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Faezeh (Verified Customer) (01-29-2023)

Although it was hard to get, it shows the expectable result.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549 cell line

AGO2 Antibody Western Blot validation (1:1000 dilution) in A549 cell line (Cat no:10686-1-AP)

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