Anticorps Polyclonal de lapin anti-c-Fos

• Phare
• Validé par KD/KO

c-Fos Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

28

Avis (2)

N° de cat : 26192-1-AP

Synonymes

AP 1, C FOS, Cellular oncogene fos, c-Fos, FOS, G0S7, Proto oncogene c Fos

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 26192-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26192-1-AP (c-Fos antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26192-1-AP (c-Fos antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26192-1-AP (c-FOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26192-1-AP (c-FOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 26192-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26192-1-AP (c-FOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7,
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 10 publications below
• IF: See 8 publications below

Informations sur le produit

26192-1-AP cible c-Fos dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: c-Fos Protéine recombinante Ag24340
• Nom complet: FOS
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC004490
• Symbole du gène: FOS
• Identification du gène (NCBI): 2353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

c-Fos, also named as FOS and G0/G1 switch regulatory protein 7, is a 380 amino acid protein, which contains 1 bZIP (basic-leucine zipper) domain and belongs to the bZIP family. c-Fos is expressed at very low levels in quiescent cells. When cells are stimulated to reenter growth, c-Fos undergo 2 waves of expression, the first one peaks 7.5 minutes following FBS induction. At this stage, the c-Fos protein is localized endoplasmic reticulum. The second wave of expression occurs at about 20 minutes after induction and peaks at 1 hour. At this stage, the c-FOS protein becomes nuclear. c-Fos is a very short-lived intracellular protein, which is very easy to degrade. The calculated molecular weight of c-Fos is 40 kDa, but Phosphorylated c-Fos protein is about 60-65 kDa. It is involved in important cellular events, including cell proliferation, differentiation and survival; genes associated with hypoxia; and angiogenesis; which makes its dysregulation an important factor for cancer development. It can also induce a loss of cell polarity and epithelial-mesenchymal transition, leading to invasive and metastatic growth in mammary epithelial cells. Expression of c-Fos is an indirect marker of neuronal activity because c-Fos is often expressed when neurons fire action potentials. Upregulation of c-Fos mRNA in a neuron indicates recent activity.

Publications

Species	Application	Title
mouse	IHC, IF	Nature; Mechanisms of stretch-mediated skin expansion at single-cell resolution.; Authors - Mariaceleste Aragona
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
mouse	IHC	Nat Commun; Ketamine activates adult-born immature granule neurons to rapidly alleviate depression-like behaviors in mice.; Authors - Radhika Rawat
mouse	IHC	Biol Psychiatry; Fighting Females: Neural and Behavioral Consequences of Social Defeat Stress in Female Mice.; Authors - Emily L Newman
human	IHC	Nat Commun; Excitatory VTA to DH projections provide a valence signal to memory circuits.; Authors - Yuan Han
mouse	IHC	Nat Commun; Activating newborn neurons suppresses depression and anxiety-like behaviors.; Authors - Elif Tunc-Ozcan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Matthias (Verified Customer) (09-10-2019)

Good Antibody, very specific.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 2000
• Cell Tissue Type: Brain tissue

c-Fos Antibody Immunohistochemistry,Immunofluorescence, validation (2000 dilution) in Brain tissue (Cat no:26192-1-AP)

FH

Elif (Verified Customer) (06-21-2019)

It works better with 2 overnight incubation in 4 degrees.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Dentate gyrus

c-Fos Antibody Immunohistochemistry, validation (1:500 dilution) in Dentate gyrus (Cat no:26192-1-AP)