Anticorps Polyclonal de lapin anti-GLUT4

GLUT4 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

25

N° de cat : 21048-1-AP

Synonymes

GLUT 4, GLUT4, SLC2A4

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Galerie de données de validation

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  WB analysis of mouse adipose using 21048-1-AP

  mouse adipose tissue were subjected to SDS PAGE followed by western blot with 21048-1-AP (GLUT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 21048-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21048-1-AP (GLUT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21048-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21048-1-AP (GLUT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 21048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21048-1-AP (GLUT4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 21048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21048-1-AP (GLUT4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 21048-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21048-1-AP (GLUT4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 21048-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21048-1-AP (GLUT4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse cerebellum using 21048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21048-1-AP (GLUT4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 21048-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21048-1-AP (GLUT4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu adipeux de souris, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu cardiaque humain, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:500
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

21048-1-AP cible GLUT4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Lasiopodomys Brandtii
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GLUT4 Protéine recombinante Ag15390
• Nom complet: solute carrier family 2 (facilitated glucose transporter), member 4
• Masse moléculaire calculée: 509 aa, 55 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC069615
• Symbole du gène: SLC2A4
• Identification du gène (NCBI): 6517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glucose transporter 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4 (SLC2A4), is a transporter protein regulating glucose transport across cell membranes in an insulin-dependent manner.

What is the molecular weight of GLUT4? Is GLUT4 post-translationally modified?

The molecular weight of GLUT4 transporter is 55 kDa. GLUT4 can be N-glycosylated, which is important for its stability and trafficking between the recycling compartment and plasma membrane (PMID: 21757715 and 22545627), and it can also be ubiquitinated and phosphorylated (PMID: 23665900).

What is the subcellular localization of GLUT4?

Glucose transporters, including GLUT4, are multiple-pass integral membrane proteins. GLUT4 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes. The localization of GLUT4 depends on stimulation with insulin - in basal conditions GLUT4 is retained intracellularly, while upon insulin stimulation it is translocated to the plasma membrane (PMID: 18570632).

What molecules can be transported by GLUT4?

Although the main substrate of GLUT4 transport is glucose, it can also transport glucosamine.

What is the tissue expression pattern of GLUT4?

GLUT4 is expressed in white and brown adipose tissue and in muscle and heart cells, where it is the main glucose transporter responsible for peripheral glucose uptake in response to insulin.

Which cell lines can be used to study insulin-dependent GLUT4 protein translocation in glucose uptake assays?

Fat and muscle cells are primarily used in glucose uptake assays because they physiologically respond to insulin (PMID: 26646194). The most commonly used cell lines are 3T3-L1 cells (murine pre-adipose fibroblasts), L6 cells (rat myoblasts), and C2C12 cells (murine myoblasts).

Publications

Species	Application	Title
mouse		Biomed Pharmacother; Qing brick tea (QBT) aqueous extract protects monosodium glutamate-induced obese mice against metabolic syndrome and involves up-regulation Transcription Factor Nuclear Factor-Erythroid 2-Related Factor 2 (Nrf2) antioxidant pathway.; Authors - Wenqi Gao
human	IHC	J Hematol Oncol; The PIK3CA E542K and E545K mutations promote glycolysis and proliferation via induction of the β-catenin/SIRT3 signaling pathway in cervical cancer.; Authors - Wei Jiang
mouse	IF	Development; LONP1-mediated mitochondrial quality control safeguards metabolic shifts in heart development.; Authors - Ke Zhao
rat		Cell Physiol Biochem; PGC-1α Participates in the Protective Effect of Chronic Intermittent Hypobaric Hypoxia on Cardiomyocytes.; Authors - Shuo Gu
rat		Food Chem Toxicol; 3-Chloro-1, 2-propanediol inhibits autophagic flux by impairment of lysosomal function in HepG2 cells.; Authors - Jing Lu
mouse		J Cell Mol Med; FGF19 protects skeletal muscle against obesity-induced muscle atrophy, metabolic derangement and abnormal irisin levels via the AMPK/SIRT-1/PGC-α pathway.; Authors - Ai Guo

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