Anticorps Polyclonal de lapin anti-LIPH

• Phare
• Validé par KD/KO

LIPH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16602-1-AP

Synonymes

AH, Lipase member H, lipase, member H, LIPH, LPD lipase related protein, LPDLR, mPA PLA1, mPA PLA1 alpha, MPAPLA1, Phospholipase A1 member B, PLA1B

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Galerie de données de validation

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  WB analysis of A549 using 16602-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16602-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16602-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16602-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 16602-1-AP

  IP Result of anti-LIPH (IP:16602-1-AP, 4ug; Detection:16602-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human pancreas using 16602-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16602-1-AP (LIPH antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 16602-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16602-1-AP (LIPH antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 16602-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 16602-1-AP (LIPH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules COLO 320, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• ELISA: See 1 publications below

Informations sur le produit

16602-1-AP cible LIPH dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: LIPH Protéine recombinante Ag9775
• Nom complet: lipase, member H
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC064941
• Symbole du gène: LIPH
• Identification du gène (NCBI): 200879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).

Publications

Species	Application	Title
human	WB	Genet Med; Loss-of-function variants in C3ORF52 result in localized autosomal recessive hypotrichosis.; Authors - Liron Malki
human	IHC	Cell Oncol (Dordr); Genomic analysis and filtration of novel prognostic biomarkers based on metabolic and immune subtypes in pancreatic cancer; Authors - Guangyu Chen
human	WB	J Cell Mol Med; LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer.; Authors - Yixiao Zhang ; KD Validated
human	IHC	Tumour Biol; Lipase member H is a novel secreted protein associated with a poor prognosis for breast cancer patients.; Authors - Meizi Cui
human	ELISA,WB,IHC	Biochem Biophys Res Commun; Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas.; Authors - Yasuhiro Seki
human	IHC	Tumour Biol; Lipase member H frequently overexpressed in human esophageal adenocarcinomas.; Authors - Hisako Ishimine