Anticorps Polyclonal de lapin anti-c-Myc

c-Myc Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10057-1-AP

Synonymes

bHLHe39, c Myc, c-Myc, MRTL, MYC, Myc proto oncogene protein, Proto oncogene c Myc, Transcription factor p64

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Galerie de données de validation

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  WB analysis of HeLa using 10057-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10057-1-AP (c-Myc antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 10057-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 10057-1-AP (c-Myc antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10057-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10057-1-AP (c-Myc antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

Dilution recommandée

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below

Informations sur le produit

10057-1-AP cible c-Myc dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: v-myc myelocytomatosis viral oncogene homolog (avian)
• Masse moléculaire calculée: 48 aa, 8 kDa
• Poids moléculaire observé: 62-65 kDa, 50 kDa
• Numéro d’acquisition GenBank: BC000917
• Symbole du gène: MYC
• Identification du gène (NCBI): 4609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. This antibody is a rabbit polyclonal antibody raised against recombinant protein of human MYC.The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC [PMID: 12189186]

Publications

Species	Application	Title
human	WB	J Hazard Mater; Proteomic analysis of cell proliferation in a human hepatic cell line (HL-7702) induced by perfluorooctane sulfonate using iTRAQ.; Authors - Ruina Cui
human	WB	Oncogenesis; SALL4 promotes gastric cancer progression through activating CD44 expression.; Authors - X Yuan
human	WB	Stem Cells Int; Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc.; Authors - Bin Chen
human	WB	PLoS One; Wogonin Has Multiple Anti-Cancer Effects by Regulating c-Myc/SKP2/Fbw7α and HDAC1/HDAC2 Pathways and Inducing Apoptosis in Human Lung Adenocarcinoma Cell Line A549.; Authors - Chen Xin-Mei XM
human	WB	PLoS One; Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway.; Authors - Tingting Yang
human	WB	Mol Biol Rep; PPARγ mediates the effects of WIN55,212-2, an synthetic cannabinoid, on the proliferation and apoptosis of the BEL-7402 hepatocarcinoma cells.; Authors - Hong Yuehui Y

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