Anticorps Monoclonal anti-PAK7

PAK7 Monoclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1H3B6

N° de cat : 66961-1-Ig

Synonymes

KIAA1264, p21 activated kinase 5, p21 activated kinase 7, PAK 5, PAK 7, PAK5, PAK7

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Galerie de données de validation

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  WB analysis of mouse brain using 66961-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66961-1-Ig (PAK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66961-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66961-1-Ig (PAK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66961-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66961-1-Ig (PAK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of Neuro-2a using 66961-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PAK7 antibody (66961-1-Ig, Clone: 1H3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of U-251 using 66961-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using PAK7 antibody (66961-1-Ig, Clone: 1H3B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de porc, tissu cérébral de rat
• Résultats positifs en IF/ICC: cellules Neuro-2a, cellules U-251

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66961-1-Ig cible PAK7 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PAK7 Protéine recombinante Ag27299
• Nom complet: p21 protein (Cdc42/Rac)-activated kinase 7
• Masse moléculaire calculée: 719 aa, 81 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC024179
• Symbole du gène: PAK7
• Identification du gène (NCBI): 57144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAK7(p21-activated kinase 7) is also named as KIAA1264, PAK5 and belongs to the STE Ser/Thr protein kinase family. It plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival.

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