Anticorps Monoclonal anti-Synaptophysin

Synaptophysin Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

13

CloneNo.

5B3F2

N° de cat : 60191-1-Ig

Synonymes

synaptophysin, SYP

---

Galerie de données de validation

• 

  WB analysis of fetal human brain using 60191-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60191-1-Ig (Synaptophysin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 60191-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60191-1-Ig (Synaptophysin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60191-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60191-1-Ig (Synaptophysin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60191-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60191-1-Ig (SYP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 60191-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60191-1-Ig (Synaptophysin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 60191-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60191-1-Ig (Synaptophysin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-12 using 60191-1-Ig

  Immunofluorescent analysis of PC-12 cells using 60191-1-Ig (Synaptophysin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

---

Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules SH-SY5Y, tissu cérébral de porc, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-12

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• FC: See 1 publications below

Informations sur le produit

60191-1-Ig cible Synaptophysin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Synaptophysin Protéine recombinante Ag11803
• Nom complet: synaptophysin
• Masse moléculaire calculée: 313 aa, 34 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC064550
• Symbole du gène: SYP
• Identification du gène (NCBI): 6855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Publications

Species	Application	Title
mouse	WB,IF	Brain Behav Immun; l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.; Authors - Danqing Xin
human	IF	Nat Protoc; Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology.; Authors - Octavian Bucur
mouse	FC	Aging Cell; Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model.; Authors - Qi Shen
mouse	WB,IF	Cell Death Dis; Phosphorylation of Parkin at serine 131 by p38 MAPK promotes mitochondrial dysfunction and neuronal death in mutant A53T α-synuclein model of Parkinson's disease.; Authors - Jialong Chen
mouse	IF	FASEB J; Silencing of circIgf1r plays a protective role in neuronal injury via regulating astrocyte polarization during epilepsy.; Authors - Lin Shao
mouse	IF,WB	Chemosphere; Arsenic exposure via drinking water during pregnancy and lactation induces autism-like behaviors in male offspring mice.; Authors - Xing Zhang

Produits associés

Synaptophysin Polyclonal antibody

17785-1-AP

VAMP2 Polyclonal antibody

10135-1-AP

Chromogranin A Polyclonal antibody

23342-1-AP

Chromogranin A Monoclonal antibody

60135-1-Ig

Nestin Polyclonal antibody

19483-1-AP

GAP43 Polyclonal antibody

16971-1-AP

NCAM1/CD56 Polyclonal antibody

14255-1-AP

NCAM1/CD56 Monoclonal antibody

60238-1-Ig