Tested Applications
| Positive WB detected in | K-562 cells, COLO 320 cells, HeLa cells, human heart tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells, mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12439-1-AP targets CREST in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3119 Product name: Recombinant human CREST protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 47-396 aa of BC034494 Sequence: CTQYQQILHRNLVYLATIADSNQNMQSLLPAPPTQNMNLGPGALTQSGSSQGLHSQGSLSDAISTGLPPSSLLQGQIGNGPSHVSMQQTAPNTLPTTSMSISGPGYSHAGPASQGVPMQGQGTIGNYVSRTNINMQSNPVSMIQQQAATSHYSSAQGGSQHYQGQSSIAMMGQGSQGSSMMGQRPMAPYRPSQQGSSQQYLGQEEYYGEQYSHSQGAAEPMGQQYYPDGHGDYAYQQSSYTEQSYDRSFEESTQHYYEGGNSQYSQQQAGYQQGAAQQQTYSQQQYPSQQSYPGQQQGYGSAQGAPSQYPGYQQGQGQQYGSYRAPQTAPSAQQQRPYGYEQGQYGNYQQ Predict reactive species |
| Full Name | synovial sarcoma translocation gene on chromosome 18-like 1 |
| Calculated Molecular Weight | 396 aa, 43 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC034494 |
| Gene Symbol | CREST |
| Gene ID (NCBI) | 26039 |
| RRID | AB_2302517 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75177 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CREST, also named as SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). CREST exists some isoforms with calulated MV 43, 40, 33 and 29 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CREST antibody 12439-1-AP | Download protocol |
| IHC protocol for CREST antibody 12439-1-AP | Download protocol |
| IP protocol for CREST antibody 12439-1-AP | Download protocol |
| WB protocol for CREST antibody 12439-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CREST/SS18L1 antibody (Cat# 12439-1-AP) has 14 citations spanning journals including Nature Neuroscience, Nature Communications, Molecular Neurodegeneration, Cell Reports, Journal of Neuroscience, Human Molecular Genetics, PLoS Genetics, Journal of Translational Medicine, Cancers, Frontiers in Veterinary Science, Histochemistry and Cell Biology, Biochemical and Biophysical Research Communications, and Learning & Memory. Research fields cover ALS/neurodegeneration, chromatin remodeling, neural development, glioblastoma, rhabdoid tumor, circadian rhythms, peripheral nerve injury, and cocaine-related synaptic plasticity.
| Species | Application | Title |
|---|---|---|
Mol Neurodegener Calcium-responsive transactivator (CREST) protein shares a set of structural and functional traits with other proteins associated with amyotrophic lateral sclerosis. | ||
Nat Commun Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. | ||
J Transl Med Disruption of the NDC80-Nek2 axis suppresses glioblastoma stemness and enhances therapeutic efficacy. | ||
Cell Rep The nBAF complex subunit CREST/SS18L1 regulates hippocampal memory processes via tyrosine 397 and histone acetyltransferase CBP. | ||
Cancers (Basel) Global Chromatin Changes Resulting from Single-Gene Inactivation-The Role of SMARCB1 in Malignant Rhabdoid Tumor. |
Reviews
What customers say
The single review rates the product 4 out of 5 stars. The antibody was tested on prostate cancer cell lines by Western Blot at a 1:500 dilution, using 5% BSA in PBST with overnight incubation at 4°C. Clear bands were detected using a Li-COR system with near-infrared secondary antibodies. Overall, the reviewer found the product performed well under these conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aktan (Verified Customer) (12-24-2019) | The product is tested in prostate cancer cell lines. Used at the given dilution factor, in 5% BSA in PBST, o/n at 4C. Bands were detected using Licor system and Licor near IR secondary antibodies.
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