CREST Polyclonal antibody, PBS Only

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  WB analysis using 12439-1-AP (same clone as 12439-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12439-1-AP (same clone as 12439-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

  WB result of CREST antibody (12439-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREST transfected HeLa cells. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 12439-1-AP (same clone as 12439-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 12439-1-AP (same clone as 12439-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

  IP result of anti-CREST (IP:12439-1-AP, 4ug; Detection:12439-1-AP 1:300) with HeLa cells lysate 3000 ug. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12439-1-AP (same clone as 12439-1-PBS)

  IP result of anti-CREST (IP:12439-1-AP, 3ug; Detection:12439-1-AP 1:1000) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12439-1-AP (same clone as 12439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 12439-1-AP (same clone as 12439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 12439-1-AP (same clone as 12439-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12439-1-AP (CREST antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 12439-1-AP (same clone as 12439-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12439-1-AP (CREST antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREST antibody (12439-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CREST Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 12439-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

SS18L1, Calcium-responsive transactivator, KIAA0693, LP2261, SS18 like protein 1

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Product Information

12439-1-PBS targets CREST in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3119

  Product name: Recombinant human CREST protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 47-396 aa of BC034494

  Sequence: CTQYQQILHRNLVYLATIADSNQNMQSLLPAPPTQNMNLGPGALTQSGSSQGLHSQGSLSDAISTGLPPSSLLQGQIGNGPSHVSMQQTAPNTLPTTSMSISGPGYSHAGPASQGVPMQGQGTIGNYVSRTNINMQSNPVSMIQQQAATSHYSSAQGGSQHYQGQSSIAMMGQGSQGSSMMGQRPMAPYRPSQQGSSQQYLGQEEYYGEQYSHSQGAAEPMGQQYYPDGHGDYAYQQSSYTEQSYDRSFEESTQHYYEGGNSQYSQQQAGYQQGAAQQQTYSQQQYPSQQSYPGQQQGYGSAQGAPSQYPGYQQGQGQQYGSYRAPQTAPSAQQQRPYGYEQGQYGNYQQ

• Full Name: synovial sarcoma translocation gene on chromosome 18-like 1
• Calculated Molecular Weight: 396 aa, 43 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC034494
• Gene Symbol: CREST
• Gene ID (NCBI): 26039
• RRID: AB_2302517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75177
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CREST, also named as SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). CREST exists some isoforms with calulated MV 43, 40, 33 and 29 kDa.