Tested Applications
| Positive WB detected in | mouse brain tissue, human heart tissue, mouse eye tissue, human skeletal muscle tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15684-1-AP targets REEP2 in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8186 Product name: Recombinant human REEP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 70-252 aa of BC006218 Sequence: IAFVIWLLSPYTKGSSVLYRKFVHPTLSNKEKEIDEYITQARDKSYETMMRVGKRGLNLAANAAVTAAAKGVLSEKLRSFSMQDLTLIRDEDALPLQRPDGRLRPSPGSLLDTIEDLGDDPALSLRSSTNPADSRTEASEDDMGDKAPKRAKPIKKAPKAEPLASKTLKTRPKKKTSGGGDSA Predict reactive species |
| Full Name | receptor accessory protein 2 |
| Calculated Molecular Weight | 252 aa, 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC006218 |
| Gene Symbol | REEP2 |
| Gene ID (NCBI) | 51308 |
| RRID | AB_2238326 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BRK0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for REEP2 antibody 15684-1-AP | Download protocol |
| IF protocol for REEP2 antibody 15684-1-AP | Download protocol |
| IHC protocol for REEP2 antibody 15684-1-AP | Download protocol |
| IP protocol for REEP2 antibody 15684-1-AP | Download protocol |
| WB protocol for REEP2 antibody 15684-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The REEP2 Polyclonal antibody (Cat# 15684-1-AP) has 16 citations published in journals including Nature, Cell Metabolism, Cell Research, Molecular Psychiatry, Current Biology, EMBO Reports, Advanced Science, Molecular Neurodegeneration, Human Molecular Genetics, Neurobiology of Aging, iScience, Brain Research, and Research Square. Research fields span endoplasmic reticulum biology, ER-phagy, lipid homeostasis, apoptosis, neurodegeneration (Alzheimer's, Parkinson's, hereditary spastic paraplegia), autophagy, axonal architecture, and organelle inheritance.
| Species | Application | Title |
|---|---|---|
Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. | ||
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Mol Neurodegener Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency | ||
Adv Sci (Weinh) Pericentrosomal Redistribution of the Endoplasmic Reticulum Ensures Organelle Symmetric Inheritance and Mitotic Progression |
Reviews
What customers say
The single review for 15684-1-AP is a 5-star rating. The reviewer used the antibody for Western Blot and Immunofluorescence on mouse brain tissue and reported excellent performance, describing it as a very clean Reep2 antibody with almost no background. Overall, the feedback highlights strong specificity and low background in both WB and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wanxia (Verified Customer) (07-16-2019) | Very good Reep2 antibody for WB and IF. It is so clean, almost no background.
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