Tested Applications
| Positive WB detected in | human heart tissue, human placenta tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human appendicitis tissue, human placenta tissue |
| Positive FC (Intra) detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16216-1-AP targets HBB in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9216 Product name: Recombinant human HBB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC007075 Sequence: MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH Predict reactive species |
| Full Name | hemoglobin, beta |
| Calculated Molecular Weight | 147 aa, 16 kDa |
| Observed Molecular Weight | 13-16 kDa |
| GenBank Accession Number | BC007075 |
| Gene Symbol | HBB |
| Gene ID (NCBI) | 3043 |
| RRID | AB_10598329 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P68871 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HBB antibody 16216-1-AP | Download protocol |
| IF protocol for HBB antibody 16216-1-AP | Download protocol |
| IHC protocol for HBB antibody 16216-1-AP | Download protocol |
| IP protocol for HBB antibody 16216-1-AP | Download protocol |
| WB protocol for HBB antibody 16216-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HBB Polyclonal Antibody (Cat# 16216-1-AP) has 16 citations spanning journals including Circulation, Nature Communications, EBioMedicine, Haematologica, EMBO Reports, Stem Cells, eLife, and JBC. Research fields cover erythroid biology and hemoglobin regulation, vascular development, hematopoiesis, heme biosynthesis, liver disease, hepatocellular carcinoma, ovarian cancer, blood-brain barrier integrity, and osteoarthritis. Applications cited include WB, IHC, and IF across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Circulation Sickle Trait and Alpha Thalassemia Increase NOS-Dependent Vasodilation of Human Arteries Through Disruption of Endothelial Hemoglobin-eNOS Interactions | ||
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
EBioMedicine Proteomic profiling and molecular reclassification of high-grade serous ovarian cancer identifies prognostic subtypes and immunotherapy biomarkers. | ||
Haematologica Inclusion of a short hairpin RNA targeting BCL11A into a β-globin expressing vector allows concurrent synthesis of curative adult and fetal hemoglobin | ||
Haematologica Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis. | ||
Reviews
What customers say
The single review for this product is highly positive, rated 5 out of 5. The reviewer used a 1:1000 dilution and reported the antibody "works good" across multiple applications, including Western Blot, Immunofluorescence, and Immunoprecipitation, when applied to HSCs (hematopoietic stem cells). Overall, the feedback indicates reliable performance and broad application compatibility.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Trinath (Verified Customer) (05-13-2026) | WORKS GOOD
|
