Tested Applications
| Positive WB detected in | rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | rat brain tissue |
| Positive IF/ICC detected in | PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25859-1-AP targets TH in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine, zebrafish, gerbil |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23075 Product name: Recombinant human TH protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-260 aa of BC104967 Sequence: MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLER Predict reactive species |
| Full Name | tyrosine hydroxylase |
| Calculated Molecular Weight | 528 aa, 59 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC104967 |
| Gene Symbol | TH |
| Gene ID (NCBI) | 7054 |
| RRID | AB_2716568 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07101 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TH antibody 25859-1-AP | Download protocol |
| IHC protocol for TH antibody 25859-1-AP | Download protocol |
| IP protocol for TH antibody 25859-1-AP | Download protocol |
| WB protocol for TH antibody 25859-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Tyrosine Hydroxylase (TH) antibody (Cat# 25859-1-AP) has 320 total citations. These appear in high-impact journals including Cell, Nature Communications, Neuron, Nature Cell Biology, Journal of Clinical Investigation, Science Advances, and Advanced Science, among over 80 journals. Associated research fields predominantly include Parkinson's disease, neurodegeneration, neuroinflammation, dopaminergic signaling, ferroptosis, gut-brain axis, and cardiovascular neuroscience.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Identifying genetic targets in clinical subtypes of Parkinson's disease for optimizing pharmacological treatment strategies | ||
Cell Excessive epithelial mechanosensation drives nociceptive innervation and chronic bladder pain via the PIEZO1-SLC7A11-glutamate axis | ||
Adv Mater Directed Neural Stem Cells Differentiation via Signal Communication with Ni-Zn Micromotors | ||
Adv Mater Intranasal Delivery of Near-infrared and Magnetic Dual-response Nanospheres to Rapidly Produce Antidepressant-like and Cognitive Enhancement Effects | ||
Nat Cell Biol Psychological stress-induced ALKBH5 deficiency promotes tumour innervation and pancreatic cancer via extracellular vesicle transfer of RNA | ||
Mol Neurodegener Repetitive transcranial magnetic stimulation alleviates motor impairment in Parkinson's disease: association with peripheral inflammatory regulatory T-cells and SYT6 |
Reviews
What customers say
All 7 reviews rate this Tyrosine Hydroxylase antibody 5 stars. Users report strong, specific signal across immunofluorescence (1:200–1:5000), Western blot (1:2000–1:20000), and immunohistochemistry (1:200). Tested successfully on mouse and rat brain tissue, mouse liver, and human iPSC-derived dopaminergic neurons and neural progenitor cells. Several reviewers noted high specificity with overnight 4°C incubation for IF, and one suggested further dilution was possible for IHC. Overall, the antibody is described as reliable and well-performing across multiple applications and species.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alice (Verified Customer) (09-11-2026) | I used this antibody for quality check on our dopaminergique neurons derived from iPSC. It works very well at 1:500 dilution.
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Kamal (Verified Customer) (01-22-2026) | Antibody works when diluted in 1XTBST @ 1:20000.
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Cristine (Verified Customer) (11-09-2025) | The Tyrosine Hydroxylase antibody worked very well and with high specificity at the dilution of 1:500 after overnight incubation at 4oC in immunocytochemistry assays of neural progenitor cells and dopaminergic neurons derived from human induced pluripotent stem cells.
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Kenzo (Verified Customer) (01-05-2024) | This antibody worked well for immunolabeling on mouse brain tissue sections
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Raphael (Verified Customer) (08-02-2021) | This antibody is good for western blot. The dilution at 1/1000 was too strong but at 1/2000 it works perfectly.
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Teresa (Verified Customer) (11-03-2017) | Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval
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Teresa (Verified Customer) (10-16-2017) | Signal was strong and specific. Could probably dilute further.
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