Tested Applications
| Positive WB detected in | A431 cells, HEK-293T cells, HT-29 cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human cervical cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:600-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30119-1-AP targets RIF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32422 Product name: Recombinant human RIF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2277-2472 aa of NM_001177663.1 Sequence: SEMAKESIPCPTESVYPPLVNCVAPVDIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKTRGLEEIPVFDISEKTVNGIENKSLSPDEERLVSDIIDPVALEIPLSKNLLAQISALALQLDSEDLHNYSGSQLFEMHEKLSCMANSVIKNLQSRWRSPSHENSI Predict reactive species |
| Full Name | RAP1 interacting factor homolog (yeast) |
| Calculated Molecular Weight | 274 kDa |
| Observed Molecular Weight | 300 kDa |
| GenBank Accession Number | NM_001177663.1 |
| Gene Symbol | RIF1 |
| Gene ID (NCBI) | 55183 |
| RRID | AB_2935516 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5UIP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RIF1 (Rap1-interacting factor 1) is a multifunctional chromosome stability protein implicated in controlling DNA replication and repair. Human RIF1 can interact with protein phosphatase 1 (PP1) and the interaction is crucial for controlling DNA replication by limiting phosphorylation of the MCM complex (PMID: 28077461). RIF1 also acts with PP1 to protect nascent DNA from overdegradation at stalled replication forks (PMID: 31141682). RIF1 promotes non-homologous end joining (NHEJ) in double strand break (DSB) repair (PMID: 30443189). Research studies show that RIF1 is overexpressed in breast cancer tissues and is upgraded in NSCLC tissues (PMID: 30237512).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RIF1 antibody 30119-1-AP | Download protocol |
| IHC protocol for RIF1 antibody 30119-1-AP | Download protocol |
| WB protocol for RIF1 antibody 30119-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RIF1 polyclonal antibody (Cat# 30119-1-AP) has 1 citation to date. It was cited in Molecular Cancer (IF 42.2), a study investigating how ACOX2 destabilizes the MRE11-RAD50-NBS1 complex to boost anticancer immunity via the cGAS-STING pathway in clear cell renal cell carcinoma. The antibody was used for Western blot and immunofluorescence in human samples. The associated research fields include DNA damage repair, tumor immunology, and renal cell carcinoma biology.
| Species | Application | Title |
|---|---|---|
Mol Cancer ACOX2 destabilizes the MRE11-RAD50-NBS1 complex and boosts anticancer immunity via the cGAS-STING pathway in clear cell renal cell carcinoma. |
