Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, HEK-293T cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse liver tissue, human colon cancer tissue, human liver cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue, mouse epididymis tissue |
| Positive IF/ICC detected in | MCF-7 cells, HepG2 cells, HeLa cells, T-47D cells, mouse testis tissue |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51067-2-AP targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, rabbit, chicken, zebrafish, hamster, sheep, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Calculated Molecular Weight | 781 aa, 86 kDa |
| Observed Molecular Weight | 92 kDa |
| GenBank Accession Number | BC058926 |
| Gene Symbol | Beta Catenin |
| Gene ID (NCBI) | 1499 |
| ENSEMBL Gene ID | ENSG00000168036 |
| RRID | AB_2086128 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35222 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| IF protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| IHC protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| IP protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| WB protocol for Beta Catenin antibody 51067-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The 51067-2-AP Beta Catenin Polyclonal antibody has 1,353 total citations. Citations appear in journals including Signal Transduction and Targeted Therapy, Nature Communications, Molecular Cancer, Cell Metabolism, Nature Metabolism, Hepatology, Journal of Experimental & Clinical Cancer Research, Cell Death & Disease, Advanced Science, Autophagy, ACS Nano, and Cancer Letters. Research fields span oncology (colorectal, pancreatic, liver, lung, breast cancers), Wnt/β-catenin signaling, bone biology, gut microbiome, stem cell biology, drug resistance, EMT, autophagy, and ferroptosis.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin | ||
Signal Transduct Target Ther Gut dysbiosis conveys psychological stress to activate LRP5/β-catenin pathway promoting cancer stemness | ||
Signal Transduct Target Ther Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivation. | ||
J Hematol Oncol The PIK3CA E542K and E545K mutations promote glycolysis and proliferation via induction of the β-catenin/SIRT3 signaling pathway in cervical cancer. | ||
Imeta The interplay between tissue-resident microbiome and host proteins by integrated multi-omics during progression of colorectal adenoma to carcinoma. | ||
Mol Cancer Transcriptional landscape and predictive potential of long noncoding RNAs in peritoneal recurrence of gastric cancer |
Reviews
What customers say
Customers report this antibody performs reliably for both Western blotting and immunofluorescence. Recommended dilutions include 1:1000–1:2000 for WB and 1:100–1:300 for IF. It has been successfully used on bone marrow MSCs and primary neonatal rat cardiomyocytes, working well after ice-cold methanol or 4% PFA fixation with 3% BSA blocking. One user noted a 1-hour room-temperature incubation for primary and secondary antibodies. Overall ratings are strong (4–5 stars), with users consistently describing it as effective and easy to use across applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
kis (Verified Customer) (02-21-2025) | This antibody works well for western blotting at 1:1000 dilution for WB and 1:300 for IF.
|
Udesh (Verified Customer) (08-16-2023) | Worked well for WB and IF
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Alzbeta (Verified Customer) (03-13-2019) | This antibody was used for IF staining of beta-catenin in primary neonatal rat cardiomyocyte culture. The antibody worked well after both ice cold-methanol or 4% PFA fixation, followed by the 3%BSA block. The incubation with primary (b-catenin) and secondary antibody (aRabbit-Alexa Fluor 488 Plus) lasted 1 hr at room temperature.
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