Tested Applications
| Positive WB detected in | unboiled mouse brain tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | SH-SY5Y cells |
For optimal WB detection with 55491-1-AP, we do not recommend boiling the sample after lysis.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:750-1:3000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55491-1-AP targets VGLUT1 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 61 kDa |
| GenBank Accession Number | NM_020309 |
| Gene Symbol | VGLUT1 |
| Gene ID (NCBI) | 57030 |
| RRID | AB_2881348 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2U7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Vesicular glutamate transporter 1 (VGLUT1) is a critical protein involved in the packaging and release of the neurotransmitter glutamate in synaptic vesicles. VGLUT1 is predominantly expressed in neurons that release glutamate, the primary excitatory neurotransmitter in the central nervous system, it is a marker of glutamatergic neurons.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for VGLUT1 antibody 55491-1-AP | Download protocol |
| IF protocol for VGLUT1 antibody 55491-1-AP | Download protocol |
| IHC protocol for VGLUT1 antibody 55491-1-AP | Download protocol |
| WB protocol for VGLUT1 antibody 55491-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VGLUT1 Polyclonal antibody (Cat# 55491-1-AP) has 36 citations spanning journals including Nature Communications, Advanced Materials, ACS Nano, Cell Reports, EMBO Reports, PLoS Biology, Neurobiology of Disease, and iScience. Research fields cover neuroscience, neurodegeneration (Alzheimer's, spinocerebellar ataxia), epilepsy, spinal cord injury, chronic pain, depression, cognitive impairment, synaptic function, and glutamatergic signaling.
| Species | Application | Title |
|---|---|---|
Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
Adv Mater Directed Neural Stem Cells Differentiation via Signal Communication with Ni-Zn Micromotors | ||
Bioact Mater Construction of functional neural network tissue combining CBD-NT3-modified linear-ordered collagen scaffold and TrkC-modified iPSC-derived neural stem cells for spinal cord injury repair | ||
Nat Commun Membrane remodeling by FAM92A1 during brain development regulates neuronal morphology, synaptic function, and cognition | ||
Nat Commun Ectoparasites enhance survival by suppressing host exploration and limiting dispersal | ||
ACS Nano Small Molecule Hydrogels Loading Small Molecule Drugs from Chinese Medicine for the Enhanced Treatment of Traumatic Brain Injury |
