CoraLite® Plus 488-conjugated VGLUT1 Polyclonal antibody

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  IF Staining of mouse brain using CL488-55491

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 VGLUT1 antibody (CL488-55491) at dilution of 1:200.

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  FC experiment of SH-SY5Y using CL488-55491

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human VGLUT1 (CL488-55491) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

VGLUT1 Polyclonal Antibody for IF-P, FC (Intra)

Cat No. CL488-55491

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Synonyms

1, SLC17A7, BNPI, Brain-specific Na(+)-dependent inorganic phosphate cotransporter

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Tested Applications

• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-55491 targets VGLUT1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
• Calculated Molecular Weight: 62 kDa
• GenBank Accession Number: NM_020309
• Gene Symbol: VGLUT1
• Gene ID (NCBI): 57030
• RRID: AB_3084131
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P2U7
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Vesicular glutamate transporter 1 (VGLUT1) is a critical protein involved in the packaging and release of the neurotransmitter glutamate in synaptic vesicles. VGLUT1 is predominantly expressed in neurons that release glutamate, the primary excitatory neurotransmitter in the central nervous system, it is a marker of glutamatergic neurons.