Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells, A549 cells, HepG2 cells |
| Positive IHC detected in | human tonsillitis tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60070-1-Ig targets IL-18 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1063 Product name: Recombinant human Pre IL-18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC007461 Sequence: MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED Predict reactive species |
| Full Name | IL-18 |
| Calculated Molecular Weight | 193 aa, 22 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC007461 |
| Gene Symbol | IL-18 |
| Gene ID (NCBI) | 3606 |
| RRID | AB_2280158 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL-18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IL-18 antibody 60070-1-Ig | Download protocol |
| IF protocol for IL-18 antibody 60070-1-Ig | Download protocol |
| IHC protocol for IL-18 antibody 60070-1-Ig | Download protocol |
| WB protocol for IL-18 antibody 60070-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-18 Monoclonal antibody (5C6F8), catalog number 60070-1-Ig, has accumulated 60 citations across journals including Nature, Nature Communications, Cell Death & Disease, Journal of Neuroinflammation, International Immunopharmacology, and Brain, Behavior, and Immunity. Associated research fields include NLRP3 inflammasome activation, pyroptosis, neuroinflammation, cardiovascular disease, diabetic complications, renal injury, cancer, stroke, and pulmonary fibrosis.
| Species | Application | Title |
|---|---|---|
Nat Commun Mast cells boost anti-tumor potency of MAIT cells via inflammasome-dependent secretion of IL-18 | ||
J Adv Res Mitochondrial dysfunction drives ZBP1-mediated PANoptosis to increase the susceptibility of heart failure with preserved ejection fraction-associated atrial fibrillation. | ||
Cell Death Dis PACS-2 deficiency aggravates tubular injury in diabetic kidney disease by inhibiting ER-phagy | ||
Int J Biol Sci Cisplatin Induces Pyroptosis via Activation of MEG3/NLRP3/caspase-1/GSDMD Pathway in Triple-Negative Breast Cancer. | ||
J Neuroinflammation Targeting the RAGE-RIPK1 binding site attenuates diabetes-associated cognitive deficits |
Reviews
What customers say
The single available review for 60070-1-Ig is from Bethany Williams (January 2022), who used the antibody at a 1:500 dilution for Western Blot on mouse kidney tissue (lot 10000138). She rated it 1 star and reported that it didn't work. With only one data point, this reflects an isolated negative experience rather than a consistent pattern, so we'd recommend trying a different lot or consulting our technical support if you encounter similar issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Bethany (Verified Customer) (01-05-2022) | Didn't work.
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