"PCNA Antibodies" Comparison
View side-by-side comparison of PCNA antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, C6 cells, ROS1728 cells, NIH/3T3 cells, 3T3-L1 cells, 4T1 cells, RAW 264.7 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human gliomas tissue, human breast cancer tissue, human liver cancer tissue, human placenta tissue, human tonsillitis tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60097-1-Ig targets PCNA in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig, rabbit, zebrafish, goat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7416 Product name: Recombinant human PCNA protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 8-256 aa of BC000491 Sequence: QGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIE Predict reactive species |
| Full Name | proliferating cell nuclear antigen |
| Calculated Molecular Weight | 29 kDa/31 kDa |
| Observed Molecular Weight | 36-38 kDa |
| GenBank Accession Number | BC000491 |
| Gene Symbol | PCNA |
| Gene ID (NCBI) | 5111 |
| RRID | AB_2236728 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P12004 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PCNA antibody 60097-1-Ig | Download protocol |
| IHC protocol for PCNA antibody 60097-1-Ig | Download protocol |
| IP protocol for PCNA antibody 60097-1-Ig | Download protocol |
| WB protocol for PCNA antibody 60097-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PCNA Monoclonal antibody (10D10E11), catalog number 60097-1-Ig, has accumulated 241 citations. Publications span over 100 journals, including Science, Nature Communications, Nature Microbiology, Cell Reports, Development, FASEB Journal, and Cell Death & Differentiation. Research fields cover oncology (hepatocellular, lung, colorectal, breast, and other cancers), cardiovascular disease, liver fibrosis and regeneration, neurology, immunology, wound healing, reproductive biology, diabetes, DNA replication, ferroptosis, and developmental biology. Cited applications include WB, IHC, IF, IP, and CoIP across human, mouse, rat, pig, rabbit, and other species.
| Species | Application | Title |
|---|---|---|
Nat Biomed Eng 4D-printed adaptive hydrogel tissue expanders for ear and breast reconstruction. | ||
Nat Microbiol Oxaloacetate sensing promotes innate immune antiviral defence against influenza virus infection. | ||
Nat Commun Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice | ||
Nat Commun USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization. | ||
Nat Commun Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice |
Reviews
What customers say
All five reviews rate this PCNA antibody 5 stars. Users consistently report strong, specific signals in Western Blot and Immunofluorescence applications across mouse embryonic stem cells, U2OS cells, and human primary fibroblasts. Overnight incubation at 4 °C is the most common protocol, with dilutions ranging from 1:200 (IF) to 1:10,000 (WB). Reviewers describe the antibody as "excellent" and "really good," highlighting its specificity and reliable performance with standard blocking conditions (3% milk or BSA). No negative feedback or troubleshooting issues were mentioned.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Julia (Verified Customer) (08-12-2024) | Really good, specific antibody! Incubated over night at 4C; 1:10000 dilution in 3% milk.
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Tsimafei (Verified Customer) (08-04-2024) | Excellent antibody. Membrane was incubated at 4C, ON
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Andrea (Verified Customer) (01-11-2023) | primary antibody were incubated at 4C, ON. Antibody provide a specific signal for mouse Pcna protein
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Hasan (Verified Customer) (11-04-2022) | U2OS cells fixed with Methanol:Aceton (7:3), 20 min, 4 degrees. Blocked for 1h with 5% FBS in Triton-x 0.1% Stained with the anti-PCNA (60097-1-Ig) (1:200) O/N @4 degress
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Maria (Verified Customer) (08-07-2021) | PCNA in human primary fibroblasts in culture. Passage number 15-25. 15 ug of protein. 4-15% TGX gel. Blocking BSA 3% in PBST. Primary ab 1:1000 in BSA O/N 4ºC. Secondary ab 1:5000 HRP.
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