Tested Applications
| Positive WB detected in | MCF-7 cells, HaCaT cells, PC-3 cells, HeLa cells, T-47D cells, pig colon tissue, rat colon tissue, mouse colon tissue |
| Positive IHC-Autostainer detected in | human colon tissue |
| Positive IHC detected in | human colon tissue, human stomach cancer tissue, human lung cancer tissue, rat colon tissue, human oesophagus cancer tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
| Positive IF-Fro detected in | mouse breast cancer |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC)-AUTOSTAINER | IHC-AUTOSTAINER : 1:12500-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:51200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60187-1-Ig targets Cytokeratin 19 in WB, IHC, IHC-Autostainer, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7407 Product name: Recombinant human KRT19 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-400 aa of BC002539 Sequence: MTSYSYRQSSATSSFGGLGGGSVRFGPGVAFRAPSIHGGSGGRGVSVSSARFVSSSSSGGYGGGYGGVLTASDGLLAGNEKLTMQNLNDRLASYLDKVRALEAANGELEVKIRDWYQKQGPGPSRDYSHYYTTIQDLRDKILGATIENSRIVLQIDNARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLARTDLEMQIEGLKEELAYLKKNHEEEISTLRGQVGGQVSVEVDSAPGTDLAKILSDMRSQYEVMAEQNRKDAEAWFTSRTEELNREVAGHTEQLQMSRSEVTDLRRTLQGLEIELQSQLSMKAALEDTLAETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLEQEIATYRSLLEGQEDHYNNLSASKVL Predict reactive species |
| Full Name | keratin 19 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC002539 |
| Gene Symbol | Cytokeratin 19 |
| Gene ID (NCBI) | 3880 |
| RRID | AB_10859834 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08727 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cytokeratin 19 (CK19 or KRT19) is a type I (acidic) cytokeratin. It is an intermediate filament protein providing structural rigidity and multipurpose scaffolds in epithelial cells. CK19 is often overexpressed in various cancers (e.g., hepatocellular carcinoma [HCC], pancreatic adenocarcinoma, lung cancer) and serves as a biomarker for hepatic progenitor cells (HPCs) associated with poor prognosis in HCC patients . Additionally, CK19 expression is common in pancreatic and gastrointestinal adenocarcinomasand has been studied as a potential diagnostic and prognostic marker for pancreatic neuroendocrine tumors (PNETs), where positive CK19 expression correlates with poor prognosis. Serum CK19 fragments (e.g., CYFRA 21-1, CK19-2G2) have been investigated as tumor markers for lung and breast cancer, with preoperative levels associated with metastasis and survival.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| IF protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| IHC protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| WB protocol for Cytokeratin 19 antibody 60187-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cytokeratin 19 Monoclonal antibody (3G1E4), catalog number 60187-1-Ig, has 29 citations. These appear in journals including Nature Communications, Cell Reports, EBioMedicine, Cancer Letters, Cell Death & Disease, Drug Resistance Updates, Stem Cells, and BMC Immunology. Research fields span liver fibrosis and cholangiocarcinoma, pancreatic cancer, ferroptosis, biliary atresia, Sjögren's disease, breast and thyroid cancer, and epithelial cell biology, with applications mainly in IF, IHC, and WB.
| Species | Application | Title |
|---|---|---|
Drug Resist Updat Targeting the USP5-HSPB1 axis overcomes ferroptosis resistance in intrahepatic cholangiocarcinoma. | ||
Nat Commun Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy | ||
Nat Commun Endocrine-exocrine miR-503-322 drives aging-associated pancreatitis via targeting MKNK1 in acinar cells | ||
J Nanobiotechnology Hepatocyte-derived tissue extracellular vesicles safeguard liver regeneration and support regenerative therapy | ||
Cell Death Dis Vitamin D receptor isoform VDRA suppresses hepatocellular tumorigenesis by restricting YAP nuclear localization. | ||
Cancer Lett NEAT1 Promotes the Perineural Invasion of Pancreatic Cancer via the E2F1/GDNF Axis |
Reviews
What customers say
Both reviewers rated this antibody 5 stars. One user reported excellent Western Blot performance at 1:10,000 overnight dilution in BMEL progenitor-like cells, noting a clear band. The other confirmed reliable Immunofluorescence results at 1:300 dilution in both human and mouse liver tissue. Overall, users find the antibody effective across applications with straightforward dilution protocols.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Claudia (Verified Customer) (03-06-2026) | this antibody works well in my progenitor-like BMEl cells at 1:10000 overnight Clear band
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Kis (Verified Customer) (02-21-2025) | This antibody is working. well for IF anlysis in human and mouse liver samples.
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