Tested Applications
| Positive WB detected in | COLO 320 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60264-1-Ig targets CCT3 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0843 Product name: Recombinant human CCT3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 246-545 aa of BC006501 Sequence: EYKKGESQTDIEITREEDFTRILQMEEEYIQQLCEDIIQLKPDVVITEKGISDLAQHYLMRANITAIRRVRKTDNNRIARACGARIVSRPEELREDDVGTGAGLLEIKKIGDEYFTFITDCKDPKACTILLRGASKEILSEVERNLQDAMQVCRNVLLDPQLVPGGGASEMAVAHALTEKSKAMTGVEQWPYRAVAQALEVIPRTLIQNCGASTIRLLTSLRAKHTQENCETWGVNGETGTLVDMKELGIWEPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQGGAPDAGQE Predict reactive species |
| Full Name | chaperonin containing TCP1, subunit 3 (gamma) |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 56-60 kDa |
| GenBank Accession Number | BC006501 |
| Gene Symbol | CCT3 |
| Gene ID (NCBI) | 7203 |
| RRID | AB_2881385 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P49368 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCT3 protein (full name: chaperonin containing TCP1 subunit 3, also known as the gamma subunit of TCP-1 protein) is an important member of the TCP-1 chaperonin family. As one of the eight essential subunits of the CCT/TRiC complex, its core function is to act as a molecular chaperone that assists in the correct folding of other cellular proteins under ATP hydrolysis. It is particularly involved in the folding process of key cytoskeletal components such as actin and tubulin, while also participating in important biological activities including ciliogenesis and telomere maintenance.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CCT3 antibody 60264-1-Ig | Download protocol |
| IHC protocol for CCT3 antibody 60264-1-Ig | Download protocol |
| WB protocol for CCT3 antibody 60264-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CCT3 Monoclonal antibody (5F5A6), catalog number 60264-1-Ig, has a total of 3 citations. These appear in Journal of Clinical Investigation and Frontiers in Cell and Developmental Biology (twice). The research fields span T cell inflammation in multiple sclerosis, Usher syndrome protein interactions within the TRiC/CCT-BBS chaperonin complex, and the role of the BBS/CCT chaperonin complex in localizing the adhesion G protein-coupled receptor ADGRV1 to primary cilia bases.
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol The BBS/CCT chaperonin complex ensures the localization of the adhesion G protein-coupled receptor ADGRV1 to the base of primary cilia | ||
Front Cell Dev Biol Usher syndrome proteins ADGRV1 (USH2C) and CIB2 (USH1J) interact and share a common interactome containing TRiC/CCT-BBS chaperonins |
