Tested Applications
| Positive IF/ICC detected in | Raji cells |
| Positive FC detected in | Daudi cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65165-1-Ig targets CD86 in IF/ICC, FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
B-lymphoblastoid cell line ARH 77 Predict reactive species |
| Full Name | CD86 molecule |
| Calculated Molecular Weight | 329 aa, 38 kDa |
| GenBank Accession Number | BC040261 |
| Gene Symbol | CD86 |
| Gene ID (NCBI) | 942 |
| ENSEMBL Gene ID | ENSG00000114013 |
| RRID | AB_2881481 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P42081 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD86 antibody 65165-1-Ig | Download protocol |
| IF protocol for CD86 antibody 65165-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Human CD86 (BU63), catalog number 65165-1-Ig, has 37 total citations. The retrieved citations span journals including J Nanobiotechnology, Mater Today Bio, Int J Biol Macromol, J Agric Food Chem, Food Funct, Respir Res, Cell Oncol, Int Immunopharmacol, and FASEB J. Research fields cover diabetic wound healing, drug delivery across the blood-brain barrier, spinal cord injury repair, collagen analogs, diabetic kidney disease, lung adenocarcinoma, osteosarcoma, rosacea, and periodontal inflammation. Applications cited include flow cytometry and immunofluorescence in human and mouse samples.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Single-cell transcriptomics-guided dynamic hydrogel delivery of artemisia argyi-derived EVs relieves ER stress and promotes diabetic wound regeneration. | ||
Mater Today Bio Engineering exosome membrane disguised thermal responsive system for targeted drug delivery and controlled release across the blood-brain barrier | ||
Int J Biol Macromol A dual-pronged glycopeptide hydrogel promotes diabetic wound healing by synchronizing metabolic-angiogenic recoupling. | ||
Int J Biol Macromol Multi-time-point controlled-release methylprednisolone composite scaffolds based on clinical benchmarking for promoting spinal cord injury repair. | ||
J Agric Food Chem Novel Collagen Analogs with Multicopy Mucin-Type Sequences for Multifunctional Enhancement Properties Using SUMO Fusion Tags | ||
Food Funct Ergosterol from edible fungi: Enhancing fatty acid oxidation via CPT1A to protect against diabetic kidney disease |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer reports that the antibody works well for immunofluorescence, producing a clear signal in a high-expressing B cell lymphoma line at a 1/400 dilution. Overall, the feedback is positive, highlighting reliable performance in IF applications with strong signal quality.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Paul (Verified Customer) (02-13-2025) | Works well for immunofluorescence. Clear signal in high expressing B cell line at 1/400 dilution.
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