Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65169-1-Ig targets CD163 in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Glycoprotein preparation from hairy cell leukemia spleen Predict reactive species |
| Full Name | CD163 molecule |
| Calculated Molecular Weight | 1156 aa, 125 kDa |
| GenBank Accession Number | BC051281 |
| Gene Symbol | CD163 |
| Gene ID (NCBI) | 9332 |
| ENSEMBL Gene ID | ENSG00000177575 |
| RRID | AB_2918446 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q86VB7 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD163 antibody 65169-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Human CD163 (GHI/61) antibody (catalog 65169-1-Ig) has 2 citations. They appear in the Chinese Journal of Natural Medicines and the FASEB Journal. Both used flow cytometry on human samples. Research fields include non-small cell lung cancer metastasis and tumor-associated macrophage-induced epithelial-mesenchymal transition, and periodontal ligament inflammatory responses.
| Species | Application | Title |
|---|---|---|
Chin J Nat Med Wenxia Changfu Formula inhibits NSCLC metastasis by halting TAMs-induced epithelial-mesenchymal transition via antagonisticallymodulating CCL18. | ||
FASEB J The Synergistic Effect of Oscillatory Fluid Shear and Porphyromonas gingivalis Lipopolysaccharide Promotes the Inflammatory Response of Periodontal Ligament Cells. |
Reviews
What customers say
The single review for this product is a 5-star rating from a flow cytometry application. The user detected CD163 in THP-1 cells using this antibody with a Coralite 488 secondary, reporting clear results. The primary was used at 0.5 µg per 10⁶ cells in 100 µL suspension. The secondary was tested at 1:200, 1:500, and 1:1000 dilutions, all of which worked, with 1:1000 performing best. Overall, the reviewer found the antibody reliable and effective for their flow cytometry workflow.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Julie (Verified Customer) (11-19-2025) | This was used to detect CD163 in THP-1 cells along with Coralite 488 secondary. It showed clear results. I tested the secondary at 1:200, 1:500 and 1:1000 and all worked, the 1:1000 concentration actually being the best.
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