FITC Plus Rat IgG2a Isotype Control (2A3)

IgG2a Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

n/a

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

Publications

1

CloneNo.

2A3

Cat no : FITC-65209

Synonyms

Rat IgG2a Isotype Control, IgG2a

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Validation Data Gallery

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  FC experiment of mouse splenocytes using FITC-65209

  1X10^6 mouse splenocytes were surface stained with 0.5 ug FITC Anti-Rat IgG2a Isotype Control (FITC-65209, Clone:2A3) (red), 0.5 ug FITC Plus Anti-Mouse CD4 (FITC-65141) (green), or unstained (blue). Cells were not fixed.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

FITC-65209 targets IgG2a Isotype Control in FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgG2a Isotype Control
• RRID: AB_2883823
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The 2A3 immunoglobulin is useful as an isotype-matched control. The 2A3 immunolglobulin binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells. It is used as an isotype control for rat IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Publications

Species	Application	Title
	FC	Int Immunopharmacol; Neutrophil PPIF exacerbates lung ischemia-reperfusion injury after lung transplantation by promoting calcium overload-induced neutrophil extracellular traps formation; Authors - Wensi Wu