Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65564-1-MR targets CD73 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Pre-B leukemia cell line Predict reactive species |
| Full Name | 5'-nucleotidase, ecto (CD73) |
| Calculated Molecular Weight | 29 kDa, 63 kDa |
| GenBank Accession Number | BC015940 |
| Gene Symbol | CD73 |
| Gene ID (NCBI) | 4907 |
| RRID | AB_3670318 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P21589 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD73 antibody 65564-1-MR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Human CD73 (AD2) Mouse IgG2a Recombinant Antibody (Cat# 65564-1-MR) has 1 total citation. It appears in Advances in Wound Care (Impact Factor 5.7), published in 2026. The cited study investigated engineered mesenchymal stem cells with endogenous trehalose expression activating the NRF2-HMOX1 pathway to enhance antioxidant stress and wound healing capacity. The antibody was used for flow cytometry (FC) on human samples, placing the research field in stem cell biology, oxidative stress signaling, and wound healing.
| Species | Application | Title |
|---|---|---|
Adv Wound Care (New Rochelle) Engineered Mesenchymal Stem Cells with Endogenous Trehalose Expression Activate the NRF2-HMOX1 Pathway to Enhance Antioxidant Stress and Wound Healing Capacity. |
