Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65592-1-MR targets CD86 in WB, IF, FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
B-lymphoblastoid cell line ARH 77 Predict reactive species |
| Full Name | CD86 molecule |
| Calculated Molecular Weight | 329 aa, 38 kDa |
| GenBank Accession Number | BC040261 |
| Gene Symbol | CD86 |
| Gene ID (NCBI) | 942 |
| ENSEMBL Gene ID | ENSG00000114013 |
| RRID | AB_3670341 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P42081 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD86 antibody 65592-1-MR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Human CD86 (BU63) Mouse IgG2a Recombinant Antibody (Cat# 65592-1-MR) has 2 citations. They appear in International Journal of Nanomedicine and Journal of Periodontal Research. The research fields cover hepatocellular carcinoma immunotherapy involving pyroptosis and immunosuppressant synergy, as well as macrophage efferocytosis and tissue repair in diabetic periodontitis. Applications cited include WB, IF, and FC.
| Species | Application | Title |
|---|---|---|
Int J Nanomedicine MOF-Mediated Aloe-Emodin Delivery Enhances Hepatocellular Carcinoma Immunotherapy via Pyroptosis and Immunosuppressant Synergy | ||
J Periodontal Res Macrophage Efferocytosis Controls Tissue Repair via Mitochondrial Dynamics in Diabetic Periodontitis. |
