CoraLite®594-conjugated Serum amyloid P component Monoclonal antibody

• 

  IF Staining of human liver cancer using CL594-66084

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Serum amyloid P component antibody (CL594-66084, Clone: 1A1D10 ) at dilution of 1:200.

• 

  IF Staining of human liver cancer using CL594-66084

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Serum amyloid P component antibody (CL594-66084, Clone: 1A1D10 ) at dilution of 1:200.

• 

  IF Staining of mouse spleen using CL594-66084

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CoraLite®594 Serum amyloid P component antibody (CL594-66084, Clone: 1A1D10 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver using CL594-66084

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CoraLite®594 Serum amyloid P component antibody (CL594-66084, Clone: 1A1D10 ) at dilution of 1:200.

Serum amyloid P component Monoclonal Antibody for IF-P

Cat No. CL594-66084

Clone No.1A1D10

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF-P

Synonyms

APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein

---

Tested Applications

• Positive IF-P detected in: human liver cancer tissue, human liver tissue, mouse spleen tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66084 targets Serum amyloid P component in IF-P applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14957

  Product name: Recombinant human APCS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 20-223 aa of BC007058

  Sequence: HTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV

• Full Name: amyloid P component, serum
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC007058
• Gene Symbol: Serum amyloid P component
• Gene ID (NCBI): 325
• RRID: AB_2934715
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02743
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.