Tested Applications
| Positive WB detected in | SKOV-3 cells, A549 cells, HT-1080 cells, Jurkat cells, NCI-H1299 cells, HUVEC cells, K-562 cells, TF-1 cells, human placenta tissue, HeLa cells, HaCaT cells, PANC-1 cells, MDA-MB-231 cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66304-1-Ig targets CD47 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14132 Product name: Recombinant human CD47 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC010016 Sequence: MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPI Predict reactive species |
| Full Name | CD47 molecule |
| Calculated Molecular Weight | 323 aa, 35 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC010016 |
| Gene Symbol | CD47 |
| Gene ID (NCBI) | 961 |
| RRID | AB_2881687 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q08722 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD47 antibody 66304-1-Ig | Download protocol |
| WB protocol for CD47 antibody 66304-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD47 Monoclonal antibody (Cat# 66304-1-Ig) has 36 citations spanning journals including Adv Sci, Sci Adv, Biomaterials, J Control Release, Small, Nat Cell Biol, FASEB J, J Biol Chem, Redox Biol, Theranostics, Acta Biomater, and Front Immunol. Research fields encompass biomimetic nanomedicine, cancer immunotherapy, liver fibrosis, kidney injury, atherosclerosis, thrombogenesis, and macrophage biology, with WB and IF as the predominant applications.
| Species | Application | Title |
|---|---|---|
Exploration (Beijing) Photodynamic Biomimetic Nanoparticles Accelerate Tumor Vascular Normalization Initiation. | ||
Adv Mater Host Cell Membrane-Cloaked Nanomedicine Reprograms Endothelial Surface for Enhanced Patency in Allogeneic Vascular Grafts. | ||
Redox Biol Endothelial transferrin receptor 1 contributes to thrombogenesis through cascade ferroptosis | ||
J Nanobiotechnology Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC. | ||
Theranostics Macrophage-biomimetic nanomedicine for targeted therapy of abdominal aortic aneurysm via Nrf2/NF-κB pathway. | ||
Adv Sci (Weinh) Remodeling Collagen Microenvironment in Liver Using a Biomimetic Nano-Regulator for Reversal of Liver Fibrosis |
