Tested Applications
| Positive WB detected in | A549 cells, LPS treated U-937 cells, HEK-293 cells, Raji cells, Jurkat cells, NCI-H1299 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66310-1-Ig targets TRAF3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12185 Product name: Recombinant human TRAF3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-351 aa of BC075087 Sequence: KMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMK Predict reactive species |
| Full Name | TNF receptor-associated factor 3 |
| Calculated Molecular Weight | 568 aa, 64 kDa |
| Observed Molecular Weight | 64 kDa |
| GenBank Accession Number | BC075087 |
| Gene Symbol | TRAF3 |
| Gene ID (NCBI) | 7187 |
| RRID | AB_2881692 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13114 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRAF3 antibody 66310-1-Ig | Download protocol |
| IHC protocol for TRAF3 antibody 66310-1-Ig | Download protocol |
| WB protocol for TRAF3 antibody 66310-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRAF3 Monoclonal antibody (Cat# 66310-1-Ig) has 21 citations across journals including Autophagy, Redox Biology, Advanced Science, Cell Death & Differentiation, Cancer Biology & Medicine, Free Radical Biology and Medicine, Journal of Virology, Frontiers in Immunology, mBio, and Veterinary Microbiology. Research fields span antiviral immunity and interferon signaling, NF-κB pathway regulation, cancer (colorectal, lung, esophageal), autophagy, macrophage polarization, inflammatory bowel disease, osteoporosis, and viral replication mechanisms.
| Species | Application | Title |
|---|---|---|
Redox Biol AGR2-mediated cell-cell communication controls the antiviral immune response by promoting the thiol oxidation of TRAF3 | ||
Adv Sci (Weinh) Pirin Inhibits FAS-Mediated Apoptosis to Support Colorectal Cancer Survival | ||
Cell Death Differ TRAF3/STAT6 axis regulates macrophage polarization and tumor progression | ||
Cancer Biol Med Migration and invasion inhibitory protein inhibits M2 macrophage polarization to suppress colorectal cancer progression through the STING-NFκB2-IL10 axis. | ||
Free Radic Biol Med Virtual screening identified natural Keap1-Nrf2 PPI inhibitor alleviates inflammatory osteoporosis through Nrf2-mir214-Traf3 axis. |
