CoraLite® Plus 488-conjugated TRAF3 Monoclonal antibody

TRAF3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E3F4

Cat no : CL488-66310

Synonyms

CAP 1, CAP1, CD40 binding protein, CD40bp, CRAF1, LAP1, LMP1 associated protein 1, TRAF3

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Validation Data Gallery

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  IF Staining of HeLa using CL488-66310

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 TRAF3 antibody (CL488-66310, Clone: 1E3F4 ) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66310 targets TRAF3 in IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: TRAF3 fusion protein Ag12185
• Full Name: TNF receptor-associated factor 3
• Calculated Molecular Weight: 568 aa, 64 kDa
• GenBank Accession Number: BC075087
• Gene Symbol: TRAF3
• Gene ID (NCBI): 7187
• RRID: AB_2919309
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5.