CoraLite®488-conjugated TRAF3 Monoclonal antibody

TRAF3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1E3F4

Cat no : CL488-66310

Synonyms

CAP 1, CAP1, CD40 binding protein, CD40bp, CRAF1, LAP1, LMP1 associated protein 1, TRAF3



Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66310 targets TRAF3 in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TRAF3 fusion protein Ag12185
Full Name TNF receptor-associated factor 3
Calculated molecular weight 568 aa, 64 kDa
GenBank accession numberBC075087
Gene symbol TRAF3
Gene ID (NCBI) 7187
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

TNF receptor-associated factor 3 (TRAF3) has many alternative names including CAP-1, CD40 receptor-associated factor 1(CRAF1), CD40-binding protein(CD40BP) and LMP1-associated protein 1 (LAP1). TRAF3 regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of innate immune response. It modulates signaling pathways leading to the production of cytokines and interferon. As an essential constituent of several E3 ubiquitin-protein ligase complexes, TRAF3 promote 'Lys-63'-linked ubiquitination of target proteins. Moreover, TRAF3 undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to various stimuli and is deubiquitinated by OTUB1, OTUB2 and OTUD5.

Protocols

Product Specific Protocols
IF protocol for CL488 TRAF3 antibody CL488-66310Download protocol
Standard Protocols
Click here to view our Standard Protocols