Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, PC-3 cells |
| Positive IHC detected in | human tonsillitis tissue, human placenta tissue, human prostate cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66481-1-Ig targets B7-H3/CD276 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6006 Product name: Recombinant human CD276 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-181 aa of BC062581 Sequence: LVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYQGYPEAEVFWQDG Predict reactive species |
| Full Name | CD276 molecule |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC062581 |
| Gene Symbol | CD276 |
| Gene ID (NCBI) | 80381 |
| RRID | AB_2881847 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5ZPR3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for B7-H3/CD276 antibody 66481-1-Ig | Download protocol |
| IHC protocol for B7-H3/CD276 antibody 66481-1-Ig | Download protocol |
| WB protocol for B7-H3/CD276 antibody 66481-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The B7-H3 (CD276) antibody (Cat# 66481-1-Ig) has 22 citations across 17 journals, including Nature Communications, Cancer Letters, Journal for ImmunoTherapy of Cancer, Journal of Translational Medicine, Frontiers in Immunology, and ESMO Open. Research fields span oncology (gastric, esophageal, colorectal, lung, glioma, renal, thyroid, and cholangiocarcinoma), immunotherapy (CAR-T, bispecific antibodies, oncolytic viruses), immune escape, lipid metabolism, and vascular calcification.
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins | ||
Cancer Lett CD276 promotes CD36-mediated lipid metabolism and confers resistance to tyrosine kinase inhibitors.
| ||
J Immunother Cancer Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells. | ||
J Immunother Cancer Engineered oncolytic virus expressing B7H3-targeting BiTE enhances antitumor T-cell immune response |
Reviews
What customers say
One customer, Margarita Orozco Moreno, gave this product a 5-star rating in November 2024. She used it for immunofluorescence on PC3 cells at a 1:400 dilution, paired with an AF488-conjugated secondary antibody. The review included an image demonstrating successful staining. Overall, the feedback is positive, indicating the antibody performed well in her IF application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Margarita (Verified Customer) (11-20-2024) | Secondary antibody: AF 488
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