Tested Applications
| Positive WB detected in | HT-29 cells, T-47D cells, MCF-7 cells, COLO 320 cells |
| Positive IHC detected in | human lung cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66623-1-Ig targets CHST4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3987 Product name: Recombinant human CHST4 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 177-386 aa of BC035282 Sequence: DPSLNLHIVHLVRDPRAVFRSRERTKGDLMIDSRIVMGQHEQKLKKEDQPYYVMQVICQSQLEIYKTIQSLPKALQERYLLVRYEDLARAPVAQTSRMYEFVGLEFLPHLQTWVHNITRGKGMGDHAFHTNARDALNVSQAWRWSLPYEKVSRLQKACGDAMNLLGYRHVRSEQEQRNLLLDLLSTWTVPEQIH Predict reactive species |
| Full Name | carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4 |
| Calculated Molecular Weight | 370 aa, 43 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC035282 |
| Gene Symbol | CHST4 |
| Gene ID (NCBI) | 10164 |
| RRID | AB_2881983 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8NCG5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CHST4, also named as HEC GlcNAc6ST and GlcNAc6ST 2, is one of a major GlcNAc-6-O-sulfotransferases involved in synthesizing the L-selectin ligands mediating lymphocyte homing. CHST4 is associated with the expression of MECA-79 epitope on HEV. It has been reported that the transcripts of CHST4 can be detected in lymph node, liver, pancreas and high endothelial venules (HEVs) but at a low level in HUVECs. Besides, CHST4 is expressed at a high level in gastric and colorectal cancer patients compared with normal mucosa. (PMID:30093410, 27748735, 10330415)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CHST4 antibody 66623-1-Ig | Download protocol |
| IHC protocol for CHST4 antibody 66623-1-Ig | Download protocol |
| WB protocol for CHST4 antibody 66623-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHST4 (Carbohydrate Sulfotransferase 4) antibody, catalog number 66623-1-Ig, has 4 total citations. These appear in Genomics Proteomics Bioinformatics, Frontiers in Oncology, Journal of Leukocyte Biology, and American Journal of Translational Research. The associated research fields center on cancer biology, particularly hepatocellular carcinoma (including HBV-related and lenvatinib resistance) and hormone receptor-positive breast cancer, with applications in prognostic marker identification, immune infiltration, and miRNA-mRNA regulatory networks.
| Species | Application | Title |
|---|---|---|
Genomics Proteomics Bioinformatics miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma. | ||
J Leukoc Biol CHST4 associates with high-abundance immune infiltration in hormone receptor-positive breast cancer | ||
Front Oncol Carbohydrate Sulfotransferase 4 Inhibits the Progression of Hepatitis B Virus-Related Hepatocellular Carcinoma and Is a Potential Prognostic Marker in Several Tumors.
| ||
Am J Transl Res The role and mechanism of CHST6-mediated lactation modification in lenvatinib resistance in hepatocellular carcinoma cells. |
Reviews
What customers say
The single available review for this CHST4 antibody (used in Western Blot) rates it 3 out of 5 stars. The reviewer noted that the antibody did not perform as expected in their experiment. No additional details on dilution, tissue, or troubleshooting were provided. Overall, the limited feedback suggests the product may require optimization or may not be ideal for all WB setups.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
DAN (Verified Customer) (08-19-2019) | the CHST4 antibody doesn't work as expected
|
