Filter: AllWBIF/ICCCytometric bead array

WB analysis of HEK-293 using 66662-1-Ig (same clone as 66662-1-PBS)
WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 66662-1-Ig (same clone as 66662-1-PBS)
WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.
WB analysis using 66662-1-Ig (same clone as 66662-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.
WB analysis using 66662-1-Ig (same clone as 66662-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GM130;GOLGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66662-1-Ig (same clone as 66662-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (66662-1-Ig, Clone: 2A4F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 66662-1-Ig (same clone as 66662-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (66662-1-Ig, Clone: 2A4F11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP51313-1
Cytometric bead array standard curve of MP51313-1, GM130;GOLGA2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66662-1-PBS. Detection antibody: 66662-3-PBS. Standard:Ag1848. Range: 0.781-100 ng/mL.
Cytometric bead array standard curve of MP51313-2
Cytometric bead array standard curve of MP51313-2, GM130;GOLGA2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66662-1-PBS. Detection antibody: 66662-3-PBS. Standard:Ag1848. Range: 0.781-100 ng/mL.

Featured Product
KD/KO Validated

GOLGA2/GM130 Monoclonal antibody, PBS Only (Capture)

GOLGA2/GM130 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 66662-1-PBS

Clone No.2A4F11

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

GM130, GOLGA2, 130 kDa cis-Golgi matrix protein, 2A4F11, GM130 autoantigen

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

66662-1-PBS targets GOLGA2/GM130 as part of a matched antibody pair:

MP51313-1: 66662-1-PBS capture and 66662-3-PBS detection (validated in Cytometric bead array)

MP51313-2: 66662-1-PBS capture and 66662-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag1848 Product name: Recombinant human GM130;GOLGA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-678 aa of BC014188 Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI Predict reactive species
Full Name	golgi autoantigen, golgin subfamily a, 2
Calculated Molecular Weight	111 kDa
Observed Molecular Weight	130 kDa
GenBank Accession Number	BC014188
Gene Symbol	GM130
Gene ID (NCBI)	2801
RRID	AB_2882017
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q08379
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)