Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, pig spleen tissue, HepG2 cells, HeLa cells, HSC-T6 cells, rat liver tissue, rat spleen tissue, pig liver tissue, rabbit liver tissue |
| Positive IHC detected in | human liver cancer tissue, human renal cell carcinoma tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue, rat liver tissue |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66743-1-Ig targets HO-1/HMOX1 in WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat, pig, rabbit, hamster, sheep |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21296 Product name: Recombinant human HO-1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-288 aa of BC001491 Sequence: MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQHYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM Predict reactive species |
| Full Name | heme oxygenase (decycling) 1 |
| Calculated Molecular Weight | 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC001491 |
| Gene Symbol | HO-1 |
| Gene ID (NCBI) | 3162 |
| RRID | AB_2882091 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P09601 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Heme oxygenase (HMOX1) catalyzes the first and rate-limiting step in the degradation of heme to yield equimolar quantities of biliverdin Ixa, carbon monoxide (CO), and iron. It has 3 isoforms: HO-1 is highly inducible, whereas HO-2 and HO-3 are constitutively expressed (PMID:10194478). Heme oxygenase-1 (HO-1) is expressed in many tissues and vascular smooth muscle cells, and endothelial cells (PMID:15451051) and has been identified as an important endogenous protective factor induced in many cell types by various stimulants, such as hemolysis, infiammatory cytokines,oxidative stress, heat shock, heavy metals, and endotoxin (PMID: 11522663). And the full-length HO-1 is very unstable and susceptible to truncation that generates an inactive, soluble form (28 kDa) (James R. Reed, Pharmacology, 535-568).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
| IF protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
| IHC protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
| WB protocol for HO-1/HMOX1 antibody 66743-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The HO-1/HMOX1 Monoclonal antibody (Cat# 66743-1-Ig) has 273 total citations appearing in journals such as Nature Communications, Nature Immunology, Advanced Science, ACS Nano, Redox Biology, Free Radical Biology and Medicine, Biomaterials, Cell Death & Disease, Phytomedicine, and Oncogene. Associated research fields include ferroptosis, oxidative stress, Nrf2/HO-1 signaling, hepatocellular carcinoma, neuroprotection, cardiovascular disease, inflammation, nanomedicine, and food/nutrition science. Primary applications are Western blot, immunohistochemistry, and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Adv Mater Functionalized Nanozyme Microcapsules Targeting Deafness Prevention via Mitochondrial Homeostasis Remodeling | ||
Nat Immunol The prostacyclin receptor PTGIR is a NRF2-dependent regulator of CD8(+) T cell exhaustion. | ||
J Extracell Vesicles Engineered Mesenchymal Stem Cell-Derived Extracellular Vesicles Reverse Endothelial-Mesenchymal Transition in Atherosclerosis | ||
Autophagy Hypoxia-preconditioned dental pulp stem cells alleviate acetaminophen-induced liver failure via promoting MYC-HIF1A/HIF-1α-BNIP3-mediated mitophagy. | ||
ACS Nano Breaking the Iron Homeostasis: A "Trojan Horse" Self-Assembled Nanodrug Sensitizes Homologous Recombination Proficient Ovarian Cancer Cells to PARP Inhibition |
Reviews
What customers say
Most reviewers report strong performance for Western blot, with successful results in human cell lines, mouse liver, and mouse satellite cells using dilutions of 1:1000–1:3000 overnight at 4 °C or 2 h at room temperature. One user also confirmed good immunofluorescence results in HepG2 cells at 1:100. A single negative review noted a non-specific 70 kDa band in mouse heart tissue. Overall, the antibody is well received for detecting HO-1/Hmox1 across multiple species and applications, with the majority of users expressing satisfaction and intent to repurchase.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marina (Verified Customer) (07-24-2024) | great antibody. Incubation with primary antibody (1:3000) ON at 4C. Incubation with secondary antibody (mouse) at RT for 2h.
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Brice-Emmanuel (Verified Customer) (12-18-2023) | Does not work. Non-specific band at 70 kDa
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Ayse Tarbin (Verified Customer) (03-16-2023) | It was a gift antibody. It worked perfectly after 2h incubation at room temp. Definately I will consider to buy it.
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Hala (Verified Customer) (08-17-2021) | HO-1 expression was revealed with a donkey anti-mouse 547 red secondary antibody
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Marta (Verified Customer) (09-29-2020) | Western blotting of Hmox1 in mouse liver. Used at a 1:1000 dilution in 3% milk and TBST overnight at 4 degrees.
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