Tested Applications
| Positive WB detected in | Daudi cells, Jurkat cells, Karpas-299 cells, Ramos cells, CTLL-2 cells, pig spleen tissue |
| Positive IHC detected in | human breast cancer tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66797-1-Ig targets SOCS3 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5386 Product name: Recombinant human SOCS3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-225 aa of BC060858 Sequence: MVTHSKFPAAGMSRPLDTSLRLKTFSSKSEYQLVVNAVRKLQESGFYWSAVTGGEANLLLSAEPAGTFLIRDSSDQRHFFTLSVKTQSGTKNLRIQCEGGSFSLQSDPRSTQPVPRFDCVLKLVHHYMPPPGAPSFPSPPTEPSSEVPEQPSAQPLPGSPPRRAYYIYSGGEKIPLVLSRPLSSNVATLQHLCRKTVNGHLDSYEKVTQLPGPIREFLDQYDAPL Predict reactive species |
| Full Name | suppressor of cytokine signaling 3 |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC060858 |
| Gene Symbol | SOCS3 |
| Gene ID (NCBI) | 9021 |
| RRID | AB_2882141 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O14543 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SOCS3 antibody 66797-1-Ig | Download protocol |
| IHC protocol for SOCS3 antibody 66797-1-Ig | Download protocol |
| WB protocol for SOCS3 antibody 66797-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SOCS3 antibody (Cat# 66797-1-Ig) has 24 total citations published in journals including Cell Death Dis, Acta Pharmacol Sin, J Ethnopharmacol, Int J Mol Sci, Front Mol Biosci, Microb Pathog, J Neurosci, and iScience. Associated research fields span oncology (melanoma, hepatocellular carcinoma, renal cell carcinoma), immunology/inflammation (macrophage polarization, TLR signaling), neurology (spinal cord injury, ALS, retinal dystrophy), metabolism (hyperuricemia, obesity, diabetic nephropathy), and autoimmune diseases (rheumatoid arthritis, asthma).
| Species | Application | Title |
|---|---|---|
Cell Death Dis PTK6 inhibits autophagy to promote uveal melanoma tumorigenesis by binding to SOCS3 and regulating mTOR phosphorylation | ||
Cell Mol Biol Lett H(2)S induces apoptosis of tumors with high IDO1 expression via NR4A1-BCL-2 and SOCS3 pathways. | ||
Acta Pharmacol Sin Epac activation ameliorates tubulointerstitial inflammation in diabetic nephropathy.
| ||
Neurotherapeutics Regulatory T cells attenuate astrogliosis via IL-10/STAT3/PKC/vimentin signaling and promote neurological recovery after spinal cord injury. | ||
J Ethnopharmacol Investigation of the mechanism of Isobavachalcone in treating rheumatoid arthritis through a combination strategy of network pharmacology and experimental verification. | ||
J Ethnopharmacol Simiao San alleviates hyperuricemia and kidney inflammation by inhibiting NLRP3 inflammasome and JAK2/STAT3 signaling in hyperuricemia mice |
