Tested Applications
| Positive WB detected in | pig lung tissue, rat lung tissue |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat lung tissue, mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66833-1-Ig targets AGER/RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9657 Product name: Recombinant human AGER protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 26-340 aa of BC020669 Sequence: ITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGT Predict reactive species |
| Full Name | advanced glycosylation end product-specific receptor |
| Calculated Molecular Weight | 404 aa, 43 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC020669 |
| Gene Symbol | AGER |
| Gene ID (NCBI) | 177 |
| RRID | AB_2882176 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15109 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AGER/RAGE antibody 66833-1-Ig | Download protocol |
| IHC protocol for AGER/RAGE antibody 66833-1-Ig | Download protocol |
| WB protocol for AGER/RAGE antibody 66833-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAGE recombinant Ig antibody (Cat# 66833-1-Ig) has 14 citations. They appear in journals including Journal of Ethnopharmacology, International Immunopharmacology, Biomolecules, Cell Oncology, Cell Signaling, Genes & Immunity, Molecular Immunology, Biochemical & Biophysical Reports, IBRO Neuroscience Reports, Applied Biochemistry and Biotechnology, and Chemical Biodiversity. Research fields span nephropathy, NAFLD, cerebral ischemia, rheumatoid arthritis, gastric ulcer, Alzheimer's disease, breast cancer, pulmonary fibrosis, nasal inflammation, nerve regeneration, and polycystic ovary syndrome.
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Huanglian Jiedu Decoction ameliorates Hypertensive Nephropathy by inhibiting the AGEs-RAGE/MAPK/AP-1 axis: An integrated transcriptomics and network pharmacology study. | ||
J Ethnopharmacol Qinggan Jiangzhi Cha ameliorates NAFLD by modulating the AGE-RAGE/PRKCA/MAPK3/AP-1 signaling axis. | ||
J Ethnopharmacol Zhongfeng Decoction attenuates cerebral ischemia-reperfusion injury by inhibiting autophagy via regulating the AGE-RAGE signaling pathway | ||
J Ethnopharmacol Siwei Zangmu Xiang alleviates rheumatoid arthritis via regulating RAGE signaling pathway: Integration of UPLC-Q-TOF-MS, network pharmacology, and in vivo/in vitro studies. | ||
Int Immunopharmacol A novel triazole derivative ameliorates ethanol-induced gastric ulcer via a NOS2-centered inhibition of the AGE-RAGE pathway. | ||
Cell Oncol (Dordr) P2RY6(+) tumor-associated macrophages as a therapeutic target: suppressing tumor growth and enhancing chemosensitivity in triple-negative breast cancer. |
