Tested Applications
| Positive WB detected in | Jurkat cells, A375 cells, HeLa cells, ROS1728 cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67049-1-Ig targets MCM5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28704 Product name: Recombinant human MCM5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 388-734 aa of BC000142 Sequence: SQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK Predict reactive species |
| Full Name | minichromosome maintenance complex component 5 |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 82-88 kDa |
| GenBank Accession Number | BC000142 |
| Gene Symbol | MCM5 |
| Gene ID (NCBI) | 4174 |
| RRID | AB_2882362 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P33992 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MCM5 antibody 67049-1-Ig | Download protocol |
| WB protocol for MCM5 antibody 67049-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MCM5 Monoclonal antibody (3B7F8), catalog number 67049-1-Ig, has 3 citations. These appear in Nature Cancer, Molecular Therapy, and World Journal of Gastrointestinal Oncology. The research fields span breast cancer progression and metastasis, cisplatin resistance in head and neck squamous cell carcinoma, and anti-cancer effects in pancreatic cancer.
| Species | Application | Title |
|---|---|---|
Nat Cancer Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis. | ||
Mol Ther Long noncoding RNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5. | ||
World J Gastrointest Oncol BTNL9 exerts anti-cancer effects by inhibiting CDC20 to induce G2/M arrest in pancreatic cancer |
