MCM5 Monoclonal antibody, PBS Only

• 

  WB analysis using 67049-1-Ig (same clone as 67049-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 67049-1-Ig (same clone as 67049-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67049-1-Ig (same clone as 67049-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67049-1-Ig (MCM5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67049-1-Ig (same clone as 67049-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67049-1-Ig (MCM5 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67049-1-PBS in a different storage buffer formulation.

MCM5 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67049-1-PBS

Clone No.3B7F8

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CDC46, CDC46 homolog, MCM5, P1 CDC46

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Product Information

67049-1-PBS targets MCM5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28704

  Product name: Recombinant human MCM5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 388-734 aa of BC000142

  Sequence: SQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGVEGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGEIQHRMQRKVLYRLK

• Full Name: minichromosome maintenance complex component 5
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 82-88 kDa
• GenBank Accession Number: BC000142
• Gene Symbol: MCM5
• Gene ID (NCBI): 4174
• RRID: AB_2882362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P33992
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Minichromosome maintenance, S. Cerevisiae, homolog of 5(MCM5), one component of the complex of MCM2-7, which is the putative replicative essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The MCM complex form a hexameric ring through the interaction surfaces of two neighboring subunits.