Tested Applications
| Positive WB detected in | mouse cerebellum tissue, mouse brain tissue, fetal human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67080-1-Ig targets EPHB1 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16347 Product name: Recombinant human EPHB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 247-371 aa of BC111744 Sequence: MVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCS Predict reactive species |
| Full Name | EPH receptor B1 |
| Calculated Molecular Weight | 984 aa, 110 kDa |
| Observed Molecular Weight | 110-120 kDa |
| GenBank Accession Number | BC111744 |
| Gene Symbol | EPHB1 |
| Gene ID (NCBI) | 2047 |
| RRID | AB_2882388 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P54762 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EPHB1, also named as EPHT2, NET, HEK6 and ELK, belongs to the protein kinase superfamily, Tyr protein kinase family, and Ephrin receptor subfamily. It is a receptor for members of the ephrin-B family. EPHB1 binds to ephrin-B1, -B2 and -B3. EPHB1 may be involved in cell-cell interactions in the nervous system. EPHB1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EPHB1 antibody 67080-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EPHB1 Monoclonal antibody (1B7C12), catalog number 67080-1-Ig, has 1 total citation. The cited publication appeared in Molecular Vision (2024), a journal focused on eye biology and vision research. The study investigated how EphB1 causes retinal damage through inflammatory pathways in the retina and retinal Müller cells, placing this citation in the fields of ophthalmology, retinal neuroscience, and inflammatory signaling.
| Species | Application | Title |
|---|---|---|
Mol Vis EphB1 causes retinal damage through inflammatory pathways in the retina and retinal Müller cells |
