Tested Applications
| Positive WB detected in | HeLa cells, 4T1 cells, HEK-293 cells, MCF-7 cells, Jurkat cells |
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67199-1-Ig targets EIF4G1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, hamster |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8357 Product name: Recombinant human EIF4G1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 296-645 aa of BC007788 Sequence: IEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN Predict reactive species |
| Full Name | eukaryotic translation initiation factor 4 gamma, 1 |
| Calculated Molecular Weight | 1600 aa, 176 kDa |
| Observed Molecular Weight | 240-250 kDa |
| GenBank Accession Number | BC007788 |
| Gene Symbol | EIF4G1 |
| Gene ID (NCBI) | 1981 |
| RRID | AB_2882492 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q04637 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF4G1 antibody 67199-1-Ig | Download protocol |
| IHC protocol for EIF4G1 antibody 67199-1-Ig | Download protocol |
| WB protocol for EIF4G1 antibody 67199-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF4G1 Monoclonal antibody (2B10G8), catalog number 67199-1-Ig, has a total of 4 citations. These appear in Advanced Science, Acta Pharmacologica Sinica, Antiviral Research, and Human Mutation. The associated research fields include viral translation mechanisms (Japanese Encephalitis Virus, Enterovirus A71), pancreatic cancer progression and drug resistance via stress granules, and B cell lymphoma involving mTOR signaling and alternative polyadenylation. Applications cited include WB and IF across human, mouse, and hamster species.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) DDX3 Regulates the Cap-Independent Translation of the Japanese Encephalitis Virus via Its Interactions with PABP1 and the Untranslated Regions of the Viral Genome | ||
Acta Pharmacol Sin G3BP2 promotes tumor progression and gemcitabine resistance in PDAC via regulating PDIA3-DKC1-hENT in a stress granules-dependent manner | ||
Antiviral Res Proteomic and phosphoproteomic analysis of responses to enterovirus A71 infection reveals novel targets for antiviral and viral replication | ||
Hum Mutat Vimentin Regulates Alternative Polyadenylation and mTOR Signaling via ARVCF to Promote B Cell Lymphoma Progression. |
