Tested Applications
| Positive WB detected in | mouse brain tissue, A-673 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67203-1-Ig targets DCC-Specific in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20569 Product name: Recombinant human DCC-Specific protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 25-364 aa of NM_005215 Sequence: AHLQVTGFQIKAFTALRFLSEPSDAVTMRGGNVLLDCSAESDRGVPVIKWKKDGIHLALGMDERKQQLSNGSLLIQNILHSRHHKPDEGLYQCEASLGDSGSIISRTAKVAVAGPLRFLSQTESVTAFMGDTVLLKCEVIGEPMPTIHWQKNQQDLTPIPGDSRVVVLPSGALQISRLQPGDIGIYRCSARNPASSRTGNEAEVRILSDPGLHRQLYFLQRPSNVVAIEGKDAVLECCVSGYPPPSFTWLRGEEVIQLRSKKYSLLGGSNLLISNVTDDDSGMYTCVVTYKNENISASAELTVLVPPWFLNHPSNLYAYESMDIEFECTVSGKPVPTVNW Predict reactive species |
| Full Name | deleted in colorectal carcinoma |
| Calculated Molecular Weight | 1447 aa, 158 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | NM_005215 |
| Gene Symbol | DCC |
| Gene ID (NCBI) | 1630 |
| RRID | AB_2882496 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43146 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DCC, also named as IGDCC1, belongs to the immunoglobulin superfamily and DCC family. DCC is a receptor for netrin required for axon guidance. DCC plays a key role in the developing nervous system. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. DCC gene is a tumor suppressor gene. This antibody recognizes endogenous DCC with an apparent molecular weight of 180 kDa. As the predicted molecular mass of DCC is 158 kDa, the slower mobility is likely attributable to post-translational modification, perhaps by glycosylation at potential N-linked glycosylation sites (PMID: 7926722; 8044801).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DCC-Specific antibody 67203-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DCC-Specific Monoclonal antibody (2G4G4), catalog number 67203-1-Ig, has a total of 2 citations. These citations appear in Journal of Ethnopharmacology and Neurobiology of Stress. The research fields associated with these citations include neurobiology and ethnopharmacology, specifically involving studies on synaptic function, inflammatory responses related to PTSD, and fear memory extinction with hippocampal long-term potentiation in mice. Both studies utilized WB and IF applications with mouse species.
| Species | Application | Title |
|---|---|---|
J Ethnopharmacol Novel mechanisms of Anshen Dingzhi prescription against PTSD: Inhibiting DCC to modulate synaptic function and inflammatory responses | ||
Neurobiol Stress DCC, a potential target for controlling fear memory extinction and hippocampal LTP in male mice receiving single prolonged stress |
