Tested Applications
| Positive WB detected in | rat liver tissue, mouse liver tissue, rat pancreas tissue, mouse pancreas tissue |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67294-1-Ig targets GNMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4598 Product name: Recombinant human GNMT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-295 aa of BC032627 Sequence: MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCQRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEANWMTLDKDVPQSAEGGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRAGGLLVIDHRNYDHILSTGCAPPGKNIYYKSDLTKDVTTSVLIVNNKAHMVTLDYTVQVPGAGQDGSPGLSKFRLSYYPHCLASFTELLQAAFGGKCQHSVLGDFKPYKPGQTYIPCYFIHVLKRTD Predict reactive species |
| Full Name | glycine N-methyltransferase |
| Calculated Molecular Weight | 295 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC032627 |
| Gene Symbol | GNMT |
| Gene ID (NCBI) | 27232 |
| RRID | AB_2882559 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q14749 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GNMT antibody 67294-1-Ig | Download protocol |
| WB protocol for GNMT antibody 67294-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GNMT Monoclonal antibody (1B5E3), catalog number 67294-1-Ig, has 2 total citations. These appear in Frontiers in Oncology (gallbladder carcinoma, RNA methylation-related genes) and Cell Biochemistry and Biophysics (prostate cancer, glycine/sarcosine metabolic pathway). Both studies used human samples, with applications in IHC and WB respectively. The research fields span oncology and metabolic biology.
| Species | Application | Title |
|---|---|---|
Front Oncol Mining of RNA Methylation-Related Genes and Elucidation of Their Molecular Biology in Gallbladder Carcinoma. | ||
Cell Biochem Biophys Brucine Suppresses Malignant Progression of Prostate Cancer by Decreasing Sarcosine Accumulation via Downregulation of GNMT in the Glycine/sarcosine Metabolic Pathway |
Reviews
What customers say
One user reviewed this antibody for Western Blot on mouse liver tissue at a 1:8000 dilution. They gave it a 5-star rating and described it as a great antibody with strong signal and clean background. Overall, the feedback is positive, highlighting reliable performance and good signal-to-noise ratio in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (11-11-2022) | Great antibody for WB. Strong signal and clean background.
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