Tested Applications
| Positive WB detected in | HT-1080 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, THP-1 cells, MDA-MB-231 cells, JAR cells, K-562 cells, U-937 cells |
| Positive IHC detected in | human liver cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver tissue |
This antibody is not recommended for immunocytofluorescent assays.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67300-1-Ig targets CD107a / LAMP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, canine, bovine, goat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28869 Product name: Recombinant human CD107a protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 35-250 aa of BC021288 Sequence: NGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNT Predict reactive species |
| Full Name | lysosomal-associated membrane protein 1 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 90-120 kDa |
| GenBank Accession Number | BC021288 |
| Gene Symbol | LAMP1 |
| Gene ID (NCBI) | 3916 |
| ENSEMBL Gene ID | ENSG00000185896 |
| RRID | AB_2882564 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11279 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD107a / LAMP1 antibody 67300-1-Ig | Download protocol |
| IHC protocol for CD107a / LAMP1 antibody 67300-1-Ig | Download protocol |
| WB protocol for CD107a / LAMP1 antibody 67300-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SQSTM1 (p62) antibody (Cat# 67300-1-Ig) has 65 citations published in journals including Cell, Nature Communications, Autophagy, PNAS, Advanced Materials, Advanced Science, Free Radical Biology and Medicine, Biochemical Pharmacology, Cell Death & Disease, and International Journal of Biological Sciences. Research fields span autophagy and lysosomal biology, ferroptosis, multiple cancer types (hepatocellular, colorectal, lung, breast, glioblastoma), neurodegeneration (Parkinson's disease), inflammation and immunity, metabolic disorders, cardiovascular disease, infectious diseases, and cellular senescence.
| Species | Application | Title |
|---|---|---|
Adv Mater A Bifunctional Lysosome-Targeting Chimera Nanoplatform for Tumor-Selective Protein Degradation and Enhanced Cancer Immunotherapy | ||
Drug Resist Updat TMOD3 accelerated resistance to immunotherapy in KRAS-mutated pancreatic cancer through promoting autophagy-dependent degradation of ASCL4 | ||
Bone Res Chaperone-mediated autophagy directs a dual mechanism to balance premature senescence and senolysis to prevent intervertebral disc degeneration | ||
Autophagy Regorafenib enhances anti-PDCD1/PD-1 therapeutic efficacy in colorectal cancer by promoting SQSTM1/p62-mediated CD274/PD-L1 degradation. | ||
Autophagy Autophagy regulates cellular senescence by mediating the degradation of CDKN1A/p21 and CDKN2A/p16 through SQSTM1/p62-mediated selective autophagy in myxomatous mitral valve degeneration |
