Tested Applications
| Positive WB detected in | Jurkat cells, HepG2 cells, HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67409-1-Ig targets SEC24D in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4657 Product name: Recombinant human SEC24D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 783-1033 aa of BC035761 Sequence: TDALINFFAKSAFKAVLHQPLKVIREILVNQTAHMLACYRKNCASPSAASQLILPDSMKVLPVYMNCLLKNCVLLSRPEISTDERAYQRQLVMTMGVADSQLFFYPQLLPIHTLDVKSTMLPAAVRCSESRLSEEGIFLLANGLHMFLWLGVSSPPELIQGIFNVPSFAHINTDMTLLPEVGNPYSQQLRMIMGIIQQKRPYSMKLTIVKQREQPEMVFRQFLVEDKGLYGGSSYVDFLCCVHKEICQLLN Predict reactive species |
| Full Name | SEC24 family, member D (S. cerevisiae) |
| Calculated Molecular Weight | 1033 aa, 113 kDa |
| Observed Molecular Weight | 113 kDa |
| GenBank Accession Number | BC035761 |
| Gene Symbol | SEC24D |
| Gene ID (NCBI) | 9871 |
| RRID | AB_2882650 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O94855 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SEC24D antibody 67409-1-Ig | Download protocol |
| WB protocol for SEC24D antibody 67409-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SEC24D Monoclonal antibody (3G5G9), catalog number 67409-1-Ig, has 1 total citation. It appears in Cell Death Differentiation (2026), where it was used for CoIP in mouse samples. The associated research field involves VMP1-mediated regulation of STING trafficking and activation through CDIPT recruitment to ER membrane curvature, intersecting with innate immune signaling and vesicular transport biology.
| Species | Application | Title |
|---|---|---|
Cell Death Differ VMP1 restricts STING trafficking and activation through controlling CDIPT recruitment to ER membrane curvature. |
