SEC24D Monoclonal antibody, PBS Only

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  WB analysis of Jurkat using 67409-1-Ig (same clone as 67409-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67409-1-Ig (same clone as 67409-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67409-1-Ig (same clone as 67409-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67409-1-Ig (SEC24D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67409-1-Ig (same clone as 67409-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SEC24D antibody (67409-1-Ig, Clone: 3G5G9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67409-1-PBS in a different storage buffer formulation.

SEC24D Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67409-1-PBS

Clone No.3G5G9

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

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Product Information

67409-1-PBS targets SEC24D in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4657

  Product name: Recombinant human SEC24D protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 783-1033 aa of BC035761

  Sequence: TDALINFFAKSAFKAVLHQPLKVIREILVNQTAHMLACYRKNCASPSAASQLILPDSMKVLPVYMNCLLKNCVLLSRPEISTDERAYQRQLVMTMGVADSQLFFYPQLLPIHTLDVKSTMLPAAVRCSESRLSEEGIFLLANGLHMFLWLGVSSPPELIQGIFNVPSFAHINTDMTLLPEVGNPYSQQLRMIMGIIQQKRPYSMKLTIVKQREQPEMVFRQFLVEDKGLYGGSSYVDFLCCVHKEICQLLN

• Full Name: SEC24 family, member D (S. cerevisiae)
• Calculated Molecular Weight: 1033 aa, 113 kDa
• Observed Molecular Weight: 113 kDa
• GenBank Accession Number: BC035761
• Gene Symbol: SEC24D
• Gene ID (NCBI): 9871
• RRID: AB_2882650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O94855
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).