Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293 cells, U2OS cells, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67440-1-Ig targets PPM1D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24761 Product name: Recombinant human PPM1D protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 254-423 aa of BC060877 Sequence: NGPVRRSTVIDQIPFLAVARALGDLWSYDFFSGEFVVSPEPDTSVHTLDPQKHKYIILGSDGLWNMIPQQDAISMCQDQEEKKYLMGEHGQSCAKMLVNRALGRWRQRMLRADNTSAIVICISPEVDNQGNFTNEDELYLNLTDSPSYNSQETCVMTPSPCSTPPVKSLE Predict reactive species |
| Full Name | protein phosphatase 1D magnesium-dependent, delta isoform |
| Observed Molecular Weight | 67 kDa, 47 kDa |
| GenBank Accession Number | BC060877 |
| Gene Symbol | PPM1D |
| Gene ID (NCBI) | 8493 |
| RRID | AB_2882676 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O15297 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PPM1D antibody 67440-1-Ig | Download protocol |
| IHC protocol for PPM1D antibody 67440-1-Ig | Download protocol |
| WB protocol for PPM1D antibody 67440-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PPM1D Monoclonal antibody (2D6D5), catalog number 67440-1-Ig, has 1 total citation. The citation appears in Experimental Neurology (2025), a study investigating how PPM1D ameliorates Alzheimer's disease by promoting mitophagy. The research field is neurodegeneration, specifically Alzheimer's disease and mitochondrial quality control. The antibody was used in Western blot with mouse samples.
| Species | Application | Title |
|---|---|---|
