Tested Applications
| Positive WB detected in | pig liver tissue, COLO 320 cells, HepG2 cells, HSC-T6 cells, HT-29 cells, MCF-7 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67448-1-Ig targets BDH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7864 Product name: Recombinant human BDH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-343 aa of BC005844 Sequence: MLATRLSRPLSRLPGKTLSACDRENGARRPLLLGSTSFIPIGRRTYASAAEPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVVEIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIRRAKGRVVNISSMLGRMANPARSPYCITKFGVEAFSDCLRYEMYPLGVKVSVVEPGNFIAATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHLPGAISDMIYIR Predict reactive species |
| Full Name | 3-hydroxybutyrate dehydrogenase, type 1 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC005844 |
| Gene Symbol | BDH1 |
| Gene ID (NCBI) | 622 |
| RRID | AB_2882682 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q02338 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BDH1 antibody 67448-1-Ig | Download protocol |
| IHC protocol for BDH1 antibody 67448-1-Ig | Download protocol |
| WB protocol for BDH1 antibody 67448-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BDH1 antibody (Cat# 67448-1-Ig) has 10 citations across journals including Cell, Nature Metabolism, Acta Pharmaceutica Sinica B, MedComm, NPJ Biofilms and Microbiomes, International Journal of Molecular Sciences, PNAS Nexus, Journal of Biological Chemistry, International Journal of Biological Markers, and bioRxiv. Research fields span cancer biology (hepatocellular, lung, pancreatic, and ovarian cancers), ketone body/β-hydroxybutyrate metabolism, epigenetic regulation, and microbiome–metabolism interactions.
| Species | Application | Title |
|---|---|---|
Cell β-hydroxybutyrate enhances the metabolic fitness of CAR T cells in cancer.
| ||
Nat Metab An alternative route for β-hydroxybutyrate metabolism supports cytosolic acetyl-CoA synthesis in cancer cells | ||
Acta Pharm Sin B Parabacteroides distasonis promotes liver regeneration by increasing β-hydroxybutyric acid (BHB) production and BHB-driven STAT3 signals | ||
MedComm (2020) BDH1-mediated LRRC31 regulation dependent on histone lysine β-hydroxybutyrylation to promote lung adenocarcinoma progression
| ||
NPJ Biofilms Microbiomes Lactiplantibacillus plantarum-inoculated silage improves milk fat in dairy goats by reprogramming rumen microbiota to promote pyruvate metabolism. | ||
Int J Mol Sci Putative Role of Fatty Acid Metabolic Therapy Using Ketogenic Diet and HIF-1α Inhibition in Hepatocellular Carcinoma: Evidence from an In Vitro Study. |
