CoraLite® Plus 488-conjugated BDH1 Monoclonal antibody

BDH1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C3D4

Cat no : CL488-67448

Synonyms

BDH, BDH1, SDR9C1

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Validation Data Gallery

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  IF Staining of mouse liver using CL488-67448

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 BDH1 antibody (CL488-67448, Clone: 2C3D4 ) at dilution of 1:200.

• 

  IF Staining of mouse liver using CL488-67448

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 BDH1 antibody (CL488-67448, Clone: 2C3D4 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse liver tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67448 targets BDH1 in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BDH1 fusion protein Ag7864
• Full Name: 3-hydroxybutyrate dehydrogenase, type 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC005844
• Gene Symbol: BDH1
• Gene ID (NCBI): 622
• RRID: AB_2919476
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).