BDH1 Monoclonal antibody, PBS Only

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  WB analysis of pig liver using 67448-1-Ig (same clone as 67448-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67448-1-Ig (same clone as 67448-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67448-1-Ig (same clone as 67448-1-PBS)

  WB result of BDH1 antibody (67448-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH1 transfected MCF-7 cells. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67448-1-Ig (same clone as 67448-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 67448-1-Ig (same clone as 67448-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 67448-1-Ig (same clone as 67448-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

BDH1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67448-1-PBS

Clone No.2C3D4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

2C3D4, 3-hydroxybutyrate dehydrogenase, BDH, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, EC:1.1.1.30

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Product Information

67448-1-PBS targets BDH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7864

  Product name: Recombinant human BDH1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-343 aa of BC005844

  Sequence: MLATRLSRPLSRLPGKTLSACDRENGARRPLLLGSTSFIPIGRRTYASAAEPVGSKAVLVTGCDSGFGFSLAKHLHSKGFLVFAGCLMKDKGHDGVKELDSLNSDRLRTVQLNVCSSEEVEKVVEIVRSSLKDPEKGMWGLVNNAGISTFGEVEFTSLETYKQVAEVNLWGTVRMTKSFLPLIRRAKGRVVNISSMLGRMANPARSPYCITKFGVEAFSDCLRYEMYPLGVKVSVVEPGNFIAATSLYSPESIQAIAKKMWEELPEVVRKDYGKKYFDEKIAKMETYCSSGSTDTSPVIDAVTHALTATTPYTRYHPMDYYWWLRMQIMTHLPGAISDMIYIR

• Full Name: 3-hydroxybutyrate dehydrogenase, type 1
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC005844
• Gene Symbol: BDH1
• Gene ID (NCBI): 622
• RRID: AB_2882682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q02338
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).