PPM1B Monoclonal antibody

PPM1B Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C7H2

Cat no : 67647-1-Ig

Synonyms

PP2C beta, PP2C beta X, PP2CB, PP2CBETA, PPC2BETAX, PPM1B, Protein phosphatase 1B



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67647-1-Ig targets PPM1B in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PPM1B fusion protein Ag3871
Full Name protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Calculated Molecular Weight 479 aa, 53 kDa
Observed Molecular Weight 36, 53 kDa
GenBank Accession NumberBC012002
Gene Symbol PPM1B
Gene ID (NCBI) 5495
RRIDAB_2882847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.

Protocols

Product Specific Protocols
WB protocol for PPM1B antibody 67647-1-IgDownload protocol
IHC protocol for PPM1B antibody 67647-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols