Tested Applications
| Positive WB detected in | HEK-293 cells, ROS1728 cells, NCCIT cells, HeLa cells, MDA-MB-231 cells |
| Positive IHC detected in | rat colon tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67672-1-Ig targets DVL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26083 Product name: Recombinant human DVL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 283-416 aa of BC050454 Sequence: LGQGYPYQYPGPPPCFPPAYQDPGFSYGSGSTGSQQSEGSKSSGSTRSSRRAPGREKERRAAGAGGSGSESDHTAPSGVGSSWRERPAGQLSRGSSPRSQASATAPGLPPPHPTTKAYTVVGGPPGGPPVRELA Predict reactive species |
| Full Name | dishevelled, dsh homolog 1 (Drosophila) |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC050454 |
| Gene Symbol | DVL1 |
| Gene ID (NCBI) | 1855 |
| RRID | AB_2882868 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O14640 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway. There are two isoforms of DVL1: 75 kDa and 73 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DVL1 antibody 67672-1-Ig | Download protocol |
| IHC protocol for DVL1 antibody 67672-1-Ig | Download protocol |
| WB protocol for DVL1 antibody 67672-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DVL1 Monoclonal antibody (1A2A5), catalog number 67672-1-Ig, has a total of 1 citation. The cited publication appeared in Molecular Diversity (Impact Factor 4.3), published in February 2025. The research field is non-small cell lung cancer treatment, specifically involving the discovery of novel celastrol-based PROTACs. The antibody was used in Western Blot (WB) for human samples.
| Species | Application | Title |
|---|---|---|
Mol Divers Discovery of the novel celastrol-based PROTACs for the treatment of non-small cell lung cancer |
